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  1. 著者
  2. S
  3. 佐俣 哲郎
  1. 紀要
  2. 麻布大学雑誌
  3. 麻布大学雑誌Vol.17/18 (2008)

海生生物骨格形成に関与する酸性糖タンパク質の構造と機能の解析

https://az.repo.nii.ac.jp/records/3901
https://az.repo.nii.ac.jp/records/3901
e71cbb3a-911f-44d4-993d-edc0c8ad133a
名前 / ファイル ライセンス アクション
bull_jau_vol17-18-120.pdf bull_jau_vol17-18-120 (353.0 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2014-02-21
タイトル
タイトル 海生生物骨格形成に関与する酸性糖タンパク質の構造と機能の解析
タイトル
タイトル Structure and function analyses of the acidic glycoproteins involved in skeleton formation of marine creatures
言語 en
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
ページ属性
内容記述タイプ Other
内容記述 P(論文)
記事種別
内容記述タイプ Other
内容記述 特集
Type
内容記述タイプ Other
内容記述 FEATURE ARTICLES
著者 佐俣, 哲郎

× 佐俣, 哲郎

佐俣, 哲郎

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Samata, Tetsuro

× Samata, Tetsuro

en Samata, Tetsuro

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著者ID
000088
Author ID
000088
所属機関
麻布大学大学院環境保健学研究科
Institution or Company
Graduate School of Environmental Health, Azabu University
Abstract
内容記述タイプ Other
内容記述 Molluscan shell comprises two types of crystals, aragonite or calcite, which are arranged to form various shell microstructures. Shell of Pinctada. fucata is divided into two microstructures, an aragonitic nacreous layer and a calcitic prismatic layer. It is widely believed that organic matrix (OM) is involved in regulation and control of both the crystal polymorphism and shell microstructure (Watabe and Wilbur, 1960; Lowenstam, 1981; Weiner, 1984). Analyses of genes enoding OM-ptoteins have been carried out those in nacreous layer of pearl oyster because of its industrial value. Meanwhile, in recent years, MSI31 and Plismalin 14 have been identified in prismatic layer. The mechanism of molluscan shell formation is still unclear, even in case of P. fucata. Especially, the mechanism about the crystallization of aragonite or calcite in the alternative is left unsolved.
Samata identified and characterized the N-terminus amino acid sequences of several components (unpublished data). The present study was conducted to identify primary structure of one of these components by cDNA cloning.
Consequently, approximately between 2.5 to 3 kb nucleotide fragments were amplified and sequenced. The primary structures of the proteins encoded by these cDNA were composed of several kinds of repeat sequences rich in glycine and alanine. On the other hand, they are scarce in the acidic and basic amino acids. These features have not been reported in other OMs in molluscan shells so far. Homology analysis showed partial similarity of the amino acid sequence of the molecule with the insoluble matrix components in molluscan shells and sea urchin spicules.
Further analyses of gene expression of the cDNA clearly indicated the specific expression of these molecules in the mantle epithelium responsible for the nacreous and prismatic layers. These results imply involvement of them in shell formation of pearl oyster as insoluble matrix proteins.
書誌情報 麻布大学雑誌
en : Journal of Azabu University

巻 17/18, p. 120-124, 発行日 2009-03-31
出版者
出版者 麻布大学
Publisher
出版者 Azabu University
ISSN
収録物識別子タイプ ISSN
収録物識別子 1346-5880
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11561468
NII論文ID(NAID)
内容記述タイプ Other
内容記述 40016759574
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
text version
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
format
内容記述タイプ Other
内容記述 application/pdf
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