@article{oai:az.repo.nii.ac.jp:00003901,
 author = {佐俣, 哲郎 and Samata, Tetsuro},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {Molluscan shell comprises two types of crystals, aragonite or calcite, which are arranged to form various shell microstructures. Shell of Pinctada. fucata is divided into two microstructures, an aragonitic nacreous layer and a calcitic prismatic layer. It is widely believed that organic matrix (OM) is involved in regulation and control of both the crystal polymorphism and shell microstructure (Watabe and Wilbur, 1960; Lowenstam, 1981; Weiner, 1984). Analyses of genes enoding OM-ptoteins have been carried out those in nacreous layer of pearl oyster because of its industrial value. Meanwhile, in recent years, MSI31 and Plismalin 14 have been identified in prismatic layer. The mechanism of molluscan shell formation is still unclear, even in case of P. fucata. Especially, the mechanism about the crystallization of aragonite or calcite in the alternative is left unsolved.
 Samata identified and characterized the N-terminus amino acid sequences of several components (unpublished data). The present study was conducted to identify primary structure of one of these components by cDNA cloning.
 Consequently, approximately between 2.5 to 3 kb nucleotide fragments were amplified and sequenced. The primary structures of the proteins encoded by these cDNA were composed of several kinds of repeat sequences rich in glycine and alanine. On the other hand, they are scarce in the acidic and basic amino acids. These features have not been reported in other OMs in molluscan shells so far. Homology analysis showed partial similarity of the amino acid sequence of the molecule with the insoluble matrix components in molluscan shells and sea urchin spicules.
 Further analyses of gene expression of the cDNA clearly indicated the specific expression of these molecules in the mantle epithelium responsible for the nacreous and prismatic layers. These results imply involvement of them in shell formation of pearl oyster as insoluble matrix proteins., P(論文), 40016759574, 特集, application/pdf, FEATURE ARTICLES},
 pages = {120--124},
 title = {海生生物骨格形成に関与する酸性糖タンパク質の構造と機能の解析},
 volume = {17/18},
 year = {2009}
}