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形質転換植物を用いたダイオキシン類による汚染環境のバイオモニタリングに関する基礎研究
https://az.repo.nii.ac.jp/records/5070
https://az.repo.nii.ac.jp/records/50706973979b-f327-451f-a663-d62ac0440b34
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bull_jau_vol15-16-291.pdf (363.8 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-01-01 | |||||
| タイトル | ||||||
| タイトル | 形質転換植物を用いたダイオキシン類による汚染環境のバイオモニタリングに関する基礎研究 | |||||
| タイトル | ||||||
| タイトル | Studies on biomonitoring of dioxins in the polluted environment using transgenie plants(Research Group 2,Azabu University High-Tech Research Center Project : Study on the Biological Toxicity and Development of the Technology for the Environmental Monitoring and Remediation of Dioxins and -Related Compounds) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 記事種別 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 特集 | |||||
| Type | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | FEATURE ARTICLES | |||||
| 著者 |
其木, 茂則
× 其木, 茂則× Sonoki, Shigenori |
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| 所属機関 | ||||||
| 値 | 麻布大学環境保健学部 | |||||
| Institution or Company | ||||||
| 値 | School of Environmental Health, Azabu University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | シロイヌナズナのグルタチオンS-トランスフェラーゼ(GST)遺伝子Atlg17180の発現調節領域をゲノムに導入した形質転換タバコ培養細胞BY-2を用いて,PCB 126暴露によるレポーター遺伝子GUSの発現が初めて確認された。 | |||||
| Abstract | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Dioxins such as PCDDs, PCDFs and dioxin-like coplanar PCBs (Co-PCBs) are hard to be decomposed due to their stability and hydrophobic nature, leading to the world-wide contamination. The precise quantitative analysis of pollution levels of dioxins has been performed using a gas chromatograph equipped with the high-resolution mass spectrometry; however, this technique has the disadvantage of a high cost or a highly educated skill. In recent years it has become evident that the expression of several genes in animals was changed in response to dioxins treatment, and then this makes these genes potential candidates for use as the biomarker of exposure to dioxins. This biomarker-monitoring system will be expected to be a good substitution for the instrumental analysis as the first step analysis of dioxins in the environment. Until now, several dioxins-response genes in the genome of Arabidopsis thaliana (A. thaliand) had been found. Among them, cytochrome P450 monooxygenases, glutathione S-transferases and peroxidases which were involved in the xenobiotic transformation were found to be up-regulated by the exposure to dioxins. Especially, glutathione S-transferases genes, Atlgl7180 and Atlg78340, had the high response specificity to the exposure time and exposure chemicals. The cloning of promoter region of Atlg17180 and Atlg78340 with the promoter-analysing vector pBGWFS7.0 and the introduction of promoter region into the genome of Nicotiana tabacum BY-2 (cultured tobacco cell) has been successfully completed. In this study, the expression of reporter gene GUS in the Atlg 17180 promoter region introduced tobacco cultured cell was first observed in the response to the exposure to PCB126. | |||||
| 書誌情報 |
麻布大学雑誌 en : Journal of Azabu University 巻 15/16, p. 291-292, 発行日 2008-03-31 |
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| レポート・演題番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ2 | |||||
| 出版者 | ||||||
| 出版者 | 麻布大学 | |||||
| Publisher | ||||||
| 出版者 | Azabu University | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1346-5880 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11561558 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| text version | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| format | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
