| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2005-01-01 |
| タイトル |
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タイトル |
ブタ凍結乾燥精子の特性およびその精子からの産子作製法に関する研究 |
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タイトル |
Researches on characteristics of freeze-dried sperm and offspring production from the sperm in the pig |
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言語 |
en |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
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内容記述タイプ |
Other |
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内容記述 |
特集 |
| Type |
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内容記述タイプ |
Other |
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内容記述 |
FEATURE ARTICLES |
| 著者 |
柏崎, 直巳
紫野, 正雄
猪股, 智夫
Kahiwazaki, Naomi
Shino, Masao
Inomata, Tomo
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| 所属機関 |
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麻布大学大学院獣医学研究科 |
| 所属機関 |
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麻布大学大学院獣医学研究科 |
| 所属機関 |
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麻布大学大学院獣医学研究科 |
| Institution or Company |
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Graduate School of Veterinary Medicine, Azabu University |
| Institution or Company |
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Graduate School of Veterinary Medicine, Azabu University |
| Institution or Company |
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Graduate School of Veterinary Medicine, Azabu University |
| Abstract |
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内容記述タイプ |
Other |
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内容記述 |
Successful offspring production from freeze-dried (FD) sperm has been reported in laboratory animals but not in pigs. The integrity of the DNA in the FD sperm is reported to affect embryogenesis. Release of endonucleases from the sperm is one of the causes of induction of sperm DNA fragmentation. We examined the effects of chelating agents, which inhibit the activation of such enzymes, on DNA fragmentation in FD sperm, and on the in vitro and in vivo development of porcine oocytes. Boar spermatozoa were sonicated and suspended in buffer supplemented with 1) 50mM EGTA, 2) 50mM EDTA, 3) 10mM EDTA, or 4) no chelating agent. A fertilization medium (Pig-FM) was used as a control. Spermatozoa were put into a glass ampoule, precooled at - 40℃ for 6h, and lyophilized for 6h. The ampoules were stored at4℃ and the contents rehydrated with distilled water. The rehydrated spermatozoa were then incubated in Pig-FM at room temperature. The rate of DNA fragmentation in the control group, as assessed by the TUNEL method, increased gradually as time after rehydration elapsed. However, the rates in all experimental groups did not increase, even at 180 min. These rates were all significantly lower (P<0.05) than that of the control group. Sperm heads from all experimental and control groups were incubated for 0-60, 60-120, or 120-180 min and then injected into in vitro-matured porcine oocytes. The rate of blastocyst formation in the control group was significantly lower (P<0.05) than those in the 50-mM EGTA and 10-mM EDTA groups incubated for 120-180 min. Finally, we transferred oocytes injected sperm from 50-mM EGTA or control groups incubated for 0-60 min into recipients. One of the two recipients of the 50-mM EGTA oocytes became pregnant but aborted on day 29 after transfer. The two recipients of the control oocytes became pregnant, and one miscarried two fetuses on day 39. The results suggested that fragmentation of DNA in FD boar sperm is one of the causes of decreased in vitro development of injected oocytes to the blastocyst stage. Supplementation with 50mM EGTA in a freeze-drying buffer improves this ability. Furthermore, porcine oocytes injected with FD sperm heads were able to grow to 39-day fetuses. |
| 書誌情報 |
麻布大学雑誌
en : Journal of Azabu University
巻 11/12,
p. 114-122,
発行日 2006-03-31
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| 出版者 |
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出版者 |
麻布大学 |
| Publisher |
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出版者 |
Azabu University |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-5880 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11561468 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| text version |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| format |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
bull_jau_vol11-12-114.pdf (865.7 kB)
