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紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2004-01-01 |
| タイトル |
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タイトル |
有用細菌(乳酸菌等)の全ゲノム塩基配列決定,比較ゲノム解析および有用遺伝子の機能解明 : 第2報 |
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タイトル |
Complete genomic, comparative genomic and post-genomic analysis of lactic acid bacteria |
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en |
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jpn |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
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Other |
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FEATURE ARTICLES |
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森田, 英利
政岡, 俊夫
茅根, 士郎
和田, 恭則
有嶋, 和義
木内, 明男
坂田, 亮一
紫野, 正雄
内藤, 博之
斉藤, 康秀
西田, 利穂
印牧, 信行
滝沢, 達也
加藤, 行男
村上, 賢
福山, 正文
岸川, 正剛
久松, 伸
吉村, 哲彦
柴, 忠義
服部, 正平
Morita, Hidetoshi
Masaoka, Toshio
Chinone, Shiro
Wada, Yasunori
Arishima, Kazuyoshi
Kiuchi, Akio
Sakata, Ryoichi
Shino, Masao
Naito, Hiroyuki
Saito, Yasuhide
Nishita, Toshiho
Kanemaki, Nobuyuki
Takizawa, Tatsuya
Kato, Yukio
Murakami, Masaru
Fukuyama, Masafumi
Kishikawa, Seigo
Hisamatsu, Shin
Yoshimura, Tetsuhiko
Shiba, Tadayoshi
Hattori, Masahira
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000247 |
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000129 |
| 所属機関 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学獣医学部 |
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麻布大学健康環境科学部 |
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麻布大学健康環境科学部 |
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麻布大学健康環境科学部 |
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(財)山形県企業振興機構・生物ラジカル研究所 |
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北里大学理学部 |
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北里大学北里生命科学研究所:理研ゲノム科学総合研究センター |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Veterinary Medicine, Azabu University |
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School of Environmental Health Sciences, Azabu University |
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School of Environmental Health Sciences, Azabu University |
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School of Environmental Health Sciences, Azabu University |
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Institute for Life Support Technology, Yamagata Public Corporation for the Development of Industry |
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School of Science, Kitasato University, Institute of Life Support Technology |
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Laboratory of Genomic Information, Kitasato Institue for Life Science, Kitasato University:Human genome research group, Genomic Sciences Center, RIKEN Yokohama Institute |
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内容記述タイプ |
Abstract |
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内容記述 |
L. reuteriは, glycerol dehydratase (GD; EC 4.2.1.30)によってグリセロールからロイテリンを産生している。本菌株のグリセロール代謝は,グリセロールをGDによってロイテリンに変換し,1,3-propanediol dehydrogenase(EC 1.1.1.202)によって1,3-プロパンジオールに還元する還元反応系と,グリセロールをglycerol dehydrogenase(EC 1.1.1.6)によってジヒドロキシアセトンに変換する酸化反応系の2つの酸化還元反応系で構成されていると考えられる。従来,L. reuteriはGDによってグリセロールからロイテリンを産生していると報告されてきたが,ゲノム解析の結果から,GDではなくdiol dehydratase(DD; EC 4.2.1.28)に近い遺伝子構成であると言える。この遺伝子構造はdehydratase遺伝子を有する他のlactobacilliにおいても共通にみられた。特に, L. reuteriにおいて特徴的だったのは,L. collinoidesとL. brevisでは保存されているNADH: flavin oxidoreductase/NADH oxidase familyのドメイン構造を有する構造遺伝子が欠損している点であった。また,ロイテリン産生を考える上で重要な遺伝子であるglycerol dehydrogenaseと1,3-propanediol dehydrogenaseは, L. reuteriではpdu clusterとは異なるゲノム上の離れた位置に存在していた。つまり,dehydrataseとは別の制御系によって調節されている1,3-propanediol dehydrogenaseが,環境要因を感知し転写が抑制され,その結果,L. reuteriにおいてロイテリンが多量に産生されるものと考えられる。L. reuteriのpdu cluster下流でアデノシルコバラミン(AdoCbl)生合成系を検出した。L. brevisからは,AdoCbl生合成に関与する遺伝子群を検出することはできなかった。すなわち,L. reuteri以外の乳酸菌でAdoCbl生合成は確認されていないことから,他のlactobacilliでは,細胞外のコバラミンが存在している場合のみGDが機能するのに対し,L. reuteriは細胞外にコバラミンが存在しない場合でも,AdoCblを生合成し,ロイテリン産生が可能であると考えられる。以上,L. reuteriのpdu clusterの遺伝子構造,dehydrataseの特徴およびAdoCbl生合成系の存在が,L. reuteriのみでロイテリンが多量に産生される原因であり,L. reuteriの示す高いプロパイオテイクス効果の分子機構を解明する上で重要な知見である。 |
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内容記述タイプ |
Other |
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We attempted to determine the complete genome sequences of Lactobacillus reuteri JCM1112 (type strain) and Lactobacillus fermentum IFO3956. Production of antibacterial substances and adhesion factors for attachment to human intestinal cells largely influences contributed to the probiotic effects of lactic acid bacteria on the physiology. L. reuteri is known to produce a non-peptidic antibacterial substance, reuterin. Different from unlike bacteriocins, reuterin shows has an the antimicrobial activity against not only Grampositive bacteria but also Gram-negative bacteria, yeasts, fungi and protozoans. An operon in L. reuteri JCM1112 was found to encode the reuterin production system, whereas no orthologous counterpart was found in L. fermentum IFO3956. We analyzed a group set of genes involved in the reuterin synthesis in L. reuteri JCM1112, and found that the pdu cluster contained a class of genes responsible for polyhedral organelle formation, which were not present in the dha regulon, but lacked structural genes with the domain structure common to the NADH: flavin oxidoreductase/NADH oxidase family. In other bacteria that possess the dha regulon, the expressions of glycerol dehydratase and 1,3-propanediol dehydrogenase are subjected to synchronous transcriptional regulation to prevent the excessive production of cytotoxic reuterin. L. reuteri was shown to have a different regulatory system. Glycerol dehydrogenase conserved in L. reuteri might contribute to the maintenance of the intracellular oxidation-reduction balance when 1,3-propanediol dehydrogenase is being expressed. The pdu cluster of L. reuteri contained the structural genes homologous to propanol dehydrogenase (pduQ), propionaldehyde dehydrogenase (pduP) and propionate kinase (PduW) of S. typhimurium. This indicates that L. reuteri possesses an oxidation pathway to produce 3-hydroxypropionic acid from glycerol as well as a reduction pathway from glycerol to 1,3-propanediol via reuterin. A group of structural genes highly homologous to the genes involved in the biosynthesis of adenosylcobalamin (AdoCbl), a coenzyme of dehydratase was found in the downstream of the pdu cluster of L. reuteri. This is the first discovery for of the genes for the presence of AdoCbl biosynthesis in lactic acid bacteria. The hemABCL, cobACD and cysG genes were all found within the cob/cbi cluster in L. reuteri, while they are distantly located at different genomic loci in other bacterial species. The G+C content of the cob/cbi cluster, as well as the pdu cluster, was markedly lower than that of the other regions, which suggests that the gene cluster might have been acquired from other organisms through horizontal transmission. Thus, L. reuteri is able to transcribe almost all enzymes essential for the biosynthesis of AdoCbl from L-glutamate by a single transcription unit, indicating suggesting that L. reuteri could may produce AdoCbl more efficiently than any other bacteria. Our results also suggest that AdoCbl produced by L. reuteri might act may serve as a source of vitamin B_<12> in the intestine of humans incapable of synthesizing the substance. These genetic characteristics are specific only to L. reuteri in Lactobacillus, and are the causes for the production of reuterin in large quantities. In addition, it is considered that the efficient energy-production system and the inhibitory activity on the growth of other bacteria are importantly required for the contribution of probiotic lactic acid bacteria should contribute to the survivability in mammalian intestines, which is an important requirement for probiotic lactic acid bacteria. |
| 書誌情報 |
麻布大学雑誌
en : Journal of Azabu University
巻 9/10,
p. 250-254,
発行日 2005-03-31
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出版者 |
麻布大学 |
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出版者 |
Azabu University |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-5880 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11561468 |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
bull_jau_vol9-10-250.pdf (469.9 kB)
