| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2004-01-01 |
| タイトル |
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タイトル |
動物の生殖系列分化の高次調節に関与する遺伝子の解析 |
| タイトル |
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タイトル |
Search for genes that may be involved in higher order regulation of germ-cell differentiation in animals |
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言語 |
en |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
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内容記述タイプ |
Other |
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内容記述 |
特集 |
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内容記述タイプ |
Other |
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内容記述 |
FEATURE ARTICLES |
| 著者 |
藤谷, 英男
藤瀬, 浩
若尾, 義人
Fujitani, Hideo
Fujise, Hiroshi
Wakao, Yoshito
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- |
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- |
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000030 |
| Author ID |
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- |
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- |
| Author ID |
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000030 |
| 所属機関 |
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麻布大学大学院獣医学研究科 |
| 所属機関 |
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麻布大学大学院獣医学研究科 |
| 所属機関 |
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麻布大学大学院獣医学研究科 |
| Institution or Company |
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Graduate School of Veterinary Sciences, Azabu University |
| Institution or Company |
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Graduate School of Veterinary Sciences, Azabu University |
| Institution or Company |
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Graduate School of Veterinary Sciences, Azabu University |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
脊椎動物の生殖制御に関与する遺伝子を探索するため,有性生殖および雌性生殖をするギンブナ(Carassius auratus langsdorfi)を実験材料として,ディファレンシャルディスプレイ法により各卵巣に発現する遺伝子を比較した。その結果,生殖制御に関与する1つの候補遺伝子断片であるKD16を得た。このKD16には少なくとも5つの対立遺伝子があり,対立遺伝子特異的なプライマーによるRT-PCR法およびRT-PCR-RFLP法により,有性生殖と雌性生殖の卵巣の間では,それらの発現パターンが異なっていることがわかった。また,KD16遺伝子の発現は卵巣特異的であると思われた。さらに,5'RACE法によりKD16遺伝子のcDNA(約1.7kb)の全長を決定し,相同性検索を行ったところ,RINGドメインとB-boxドメインをもつTRIM(Tripartite motif protein family)と呼ばれる一群のタンパク質の一つと考えられた。 |
| Abstract |
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内容記述タイプ |
Other |
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内容記述 |
The Japanese silver crucian carp, a rare but ideal model animal for investigation of genes that could regulate mode of vertebrate reproduction. These fish naturally occur in normal bisexual diploid form as well as their triploid gynogenetic counterparts. Notably, the latter form reproduce themselves by parthenogenetic, or clonal, development of unreduced and unfertilized eggs, thus giving rise to genetically identical all-female offspring. The primary objective of this study was to identify and analyze genes that play crucial roles in the complicated cascade or network for induction of meiotic division to produce the gametes in almost all multicellular organisms at the final step of formation of their germ cells. In gynogenetic fish that skip meiosis and lay triploid eggs whose genomes are identical to that of somatic cells, a certain set of genes must be different from that of bisexual kin. This difference was studied by comparing genes and their expression in the ovaries of both diploid and triploid fishes. Namely, ovarian RNAs were extracted and compared between the two forms for their nucleotide sequences and subtype variation. Differential-display analyses for RT-PCR products resulting from twenty different primer sets singled out a convincing candidate gene fragment referred to as KD16 that could be linked to mechanism for regulation of germ cell differentiation. This gene was shown to have five or more alleles, each of which was expressed differently between diploid and triploid fishes and appeared to be ovary-specific. The entire stretch (approx. 1.7 kb) of cDNA of KD16 gene obtained by 5' RACE method was analyzed for its nucleotide sequence and searched for homology with data-bank sequences. The results indicate that each of five subtypes encodes the "TRIM proteins", i.e., proteins with the RING domain and the B-box domain. This implies a possible role of this gene in reproductive regulation. |
| 書誌情報 |
麻布大学雑誌
en : Journal of Azabu University
巻 9/10,
p. 133-135,
発行日 2005-03-31
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| 出版者 |
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出版者 |
麻布大学 |
| Publisher |
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出版者 |
Azabu University |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-5880 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11561468 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| text version |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| format |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
bull_jau_vol9-10-133.pdf (211.9 kB)
