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  1. 著者
  2. H
  3. 平田 強
  1. 紀要
  2. 麻布大学雑誌
  3. 麻布大学雑誌Vol.5/6 (2002)

下水中のCryptosporidium parvumオーシスト濃度と遺伝子型に関する汚染実態調査

https://az.repo.nii.ac.jp/records/4437
https://az.repo.nii.ac.jp/records/4437
5da84b24-bde3-46ae-b3ec-9f8ac334d9e2
名前 / ファイル ライセンス アクション
bull_jau_vol5-6-144.pdf bull_jau_vol5-6-144.pdf (561.9 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2002-01-01
タイトル
タイトル 下水中のCryptosporidium parvumオーシスト濃度と遺伝子型に関する汚染実態調査
タイトル
タイトル Identification of genotype of Cryptsporodium parvum oocysts in raw sewage
言語 en
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
ページ属性
内容記述タイプ Other
内容記述 P(論文)
記事種別
内容記述タイプ Other
内容記述 特集
Type
内容記述タイプ Other
内容記述 FEATURE ARTICLES
著者 平田, 強

× 平田, 強

平田, 強

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森田, 重光

× 森田, 重光

森田, 重光

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内田, 明彦

× 内田, 明彦

内田, 明彦

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Hirata, Tsuyoshi

× Hirata, Tsuyoshi

en Hirata, Tsuyoshi

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Morita, Shigemitsu

× Morita, Shigemitsu

en Morita, Shigemitsu

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Uchida, Akihiko

× Uchida, Akihiko

en Uchida, Akihiko

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著者ID
000116
Author ID
000116
所属機関
麻布大学大学院環境保健学研究科
所属機関
麻布大学大学院環境保健学研究科
所属機関
麻布大学大学院環境保健学研究科
Institution or Company
Graduate School of Environmental Health, Azabu University
Institution or Company
Graduate School of Environmental Health, Azabu University
Institution or Company
Graduate School of Environmental Health, Azabu University
Abstract
内容記述タイプ Other
内容記述 We tried to detect and identify a single Cryptosporidium oocyst in sewage and river water using PCR-RFLP or PCR direct sequence assay.
Prior to application to environmental samples, the ability of the PCR to amplify target DNA from one oocyst was examined using C. parvum HNJ-1 strain. Each oocysts isolated using glass capillary was transferred to PCR buffer containing 1% TX-100 within a PCR tube and then subjected to three freeze-thaw cycles.
Using two primer sets which amplify portion of the sequence encoding 18S rRNA or poly threonine, PCR amplification was observed in 10 of 10 samples by 18S rRNA based PCR and 47 of 53 samples by poly threonine based PCR. The results demonstrated that PCR amplification of a single oocyst was applicable in purified C. parvum oocyst.
Then, we tried to examine the applicability of single oocyst PCR to raw sewage and river water samples. PCR amplification was observed only one of 30 samples (16 samples of 18S rRNA and 14 samples of poly threonine based PCR, respectively) from raw sewage by 18S rRNA based PCR. The PCR product was sequenced and identified as 18S rRNA gene fragment of C. parvum isolate HCTX8 (genotype 2) by BLAST.
Trials were also carried out with a single oocyst from river water by poly threonine based PCR. Three of 10 samples from river water were positive. The three PCR products were analyzed by RFLP, and all RFLP patterns showed similarity to the RFLP pattern of C. parvum genotype 2. In addition, two of the three products were sequenced successfully and identified as poly threonine gene fragment of C. parvum genotype 2 by BLAST.
書誌情報 麻布大学雑誌
en : Journal of Azabu University

巻 5/6, p. 144-149, 発行日 2003-03-31
出版者
出版者 麻布大学
Publisher
出版者 Azabu University
ISSN
収録物識別子タイプ ISSN
収録物識別子 1346-5880
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11561468
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
text version
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
format
内容記述タイプ Other
内容記述 application/pdf
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