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下水中のCryptosporidium parvumオーシスト濃度と遺伝子型に関する汚染実態調査
https://az.repo.nii.ac.jp/records/4437
https://az.repo.nii.ac.jp/records/44375da84b24-bde3-46ae-b3ec-9f8ac334d9e2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bull_jau_vol5-6-144.pdf (561.9 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-01-01 | |||||
| タイトル | ||||||
| タイトル | 下水中のCryptosporidium parvumオーシスト濃度と遺伝子型に関する汚染実態調査 | |||||
| タイトル | ||||||
| タイトル | Identification of genotype of Cryptsporodium parvum oocysts in raw sewage | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 記事種別 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 特集 | |||||
| Type | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | FEATURE ARTICLES | |||||
| 著者 |
平田, 強
× 平田, 強× 森田, 重光× 内田, 明彦× Hirata, Tsuyoshi× Morita, Shigemitsu× Uchida, Akihiko |
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| 著者ID | ||||||
| 値 | 000116 | |||||
| Author ID | ||||||
| 値 | 000116 | |||||
| 所属機関 | ||||||
| 値 | 麻布大学大学院環境保健学研究科 | |||||
| 所属機関 | ||||||
| 値 | 麻布大学大学院環境保健学研究科 | |||||
| 所属機関 | ||||||
| 値 | 麻布大学大学院環境保健学研究科 | |||||
| Institution or Company | ||||||
| 値 | Graduate School of Environmental Health, Azabu University | |||||
| Institution or Company | ||||||
| 値 | Graduate School of Environmental Health, Azabu University | |||||
| Institution or Company | ||||||
| 値 | Graduate School of Environmental Health, Azabu University | |||||
| Abstract | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | We tried to detect and identify a single Cryptosporidium oocyst in sewage and river water using PCR-RFLP or PCR direct sequence assay. Prior to application to environmental samples, the ability of the PCR to amplify target DNA from one oocyst was examined using C. parvum HNJ-1 strain. Each oocysts isolated using glass capillary was transferred to PCR buffer containing 1% TX-100 within a PCR tube and then subjected to three freeze-thaw cycles. Using two primer sets which amplify portion of the sequence encoding 18S rRNA or poly threonine, PCR amplification was observed in 10 of 10 samples by 18S rRNA based PCR and 47 of 53 samples by poly threonine based PCR. The results demonstrated that PCR amplification of a single oocyst was applicable in purified C. parvum oocyst. Then, we tried to examine the applicability of single oocyst PCR to raw sewage and river water samples. PCR amplification was observed only one of 30 samples (16 samples of 18S rRNA and 14 samples of poly threonine based PCR, respectively) from raw sewage by 18S rRNA based PCR. The PCR product was sequenced and identified as 18S rRNA gene fragment of C. parvum isolate HCTX8 (genotype 2) by BLAST. Trials were also carried out with a single oocyst from river water by poly threonine based PCR. Three of 10 samples from river water were positive. The three PCR products were analyzed by RFLP, and all RFLP patterns showed similarity to the RFLP pattern of C. parvum genotype 2. In addition, two of the three products were sequenced successfully and identified as poly threonine gene fragment of C. parvum genotype 2 by BLAST. |
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| 書誌情報 |
麻布大学雑誌 en : Journal of Azabu University 巻 5/6, p. 144-149, 発行日 2003-03-31 |
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| 出版者 | ||||||
| 出版者 | 麻布大学 | |||||
| Publisher | ||||||
| 出版者 | Azabu University | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1346-5880 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11561468 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| text version | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| format | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
