| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2014-02-21 |
| タイトル |
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タイトル |
胎盤における一酸化窒素（NO）の産生とNO合成酵素（NOS）の発現 |
| タイトル |
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タイトル |
Expression of nitric oxide synthase isoforms and detection of nitric oxide in rat placenta |
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言語 |
en |
| 言語 |
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言語 |
jpn |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
nitric oxide(NO) |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
electron paramagnetic resonance(EPR) |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
|
主題 |
placenta |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
| 記事種別 |
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内容記述タイプ |
Other |
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内容記述 |
原著論文 |
| Type |
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内容記述タイプ |
Other |
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内容記述 |
ORIGINAL ARTICLE |
| 著者 |
池田, 義則
森, 智絵美
滝沢, 達也
Ikeda, Yoshinori
Mori, Chiemi
Takizawa, Tatsuya
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| 著者ID |
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- |
| 著者ID |
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- |
| 著者ID |
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000244 |
| Author ID |
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- |
| Author ID |
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- |
| Author ID |
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000244 |
| 所属機関 |
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麻布大学獣医学部動物工学研究室 |
| 所属機関 |
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麻布大学獣医学部動物工学研究室 |
| 所属機関 |
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麻布大学獣医学部動物工学研究室 |
| Institution or Company |
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Laboratory of Animal Biotechnology, School of Veterinary Medicine，Azabu University |
| Institution or Company |
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Laboratory of Animal Biotechnology, School of Veterinary Medicine，Azabu University |
| Institution or Company |
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Laboratory of Animal Biotechnology, School of Veterinary Medicine，Azabu University |
| Abstract |
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内容記述タイプ |
Other |
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内容記述 |
Nitric oxide (NO) production in the rat placenta(pars fetalis placentae and decidua basalis) was monitored and quantified by electron paramagnetic resonance (EPR) spectroscopy using an iron complex with N-(dithiocarboxy) sarcosine (Fe-DTCS) as a NO trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative reverse transcriptase-mediated polymerase chain reaction (RT-PCR) analysis. The EPR spectrum of the placenta with Fe-DTCS trapping showed a triplet signal (g = 2.038) derived from an NO-Fe-DTCS complex. The heights of the NO-Fe-DTCS (a) and MnO (b) signals were simultaneously measured to calculate the ratio (a/b) of these signal heights used for the quantification of the NO production level. Although, the ratio of the signal heights of NO-Fe-DTCS and MnO did not vary significantly with gestational stage in the fetal placenta, the ratio was markedly decreased in the maternal placenta during the last few days of gestation. At the gestational stages examined, the level of NOS 2 (iNOS) mRNA expression was significantly higher than that of NOS 3 (eNOS) mRNA expression at any given stage in the fetal placenta, and the production pattern of NO was in good accordance with the expression pattern of NOS 2 in the maternal placenta. These results suggest that NOS 2 is the predominant producer of NO in the placenta and that NOS 2-generated NO plays significant roles in the maintenance of placental functions immediately before birth. |
| 書誌情報 |
麻布大学雑誌
en : Journal of Azabu University
巻 17/18,
p. 25-30,
発行日 2009-03-31
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| 出版者 |
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出版者 |
麻布大学 |
| Publisher |
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出版者 |
Azabu University |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-5880 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11561468 |
| NII論文ID(NAID) |
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内容記述タイプ |
Other |
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内容記述 |
40016759565 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| text version |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| format |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
bull_jau_vol17-18-025 (352.0 kB)
