| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-02-25 |
| タイトル |
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|
タイトル |
Dynamic changes of intracellular zinc ion level during maturation, fertilization, activation, and development in mouse oocytes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kageyama, Atsuko
Suyama, Ayumi
Kinoshita, Ruka
Ito, Junya
Kashiwazaki, Naomi
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| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
Although it is well known that calcium oscillations are required for fertilization in all mammalian species studied to date, recent studies also showed the ejection of zinc into the extracellular milieu in a series of coordinated events, called "zinc spark," during mammalian fertilization. These results led us to the hypothesis that a zinc ion-dependent signal is important for oocyte maturation, fertilization (activation), and further embryonic development. In this study, we evaluated the amounts and localization of intracellular zinc ions during maturation, fertilization, activation, and embryonic development in mouse oocytes. Our results show that abundant zinc ions are present in both immature and mature oocytes. After in vitro fertilization, the amounts of zinc ions were dramatically decreased at the pronuclear (PN) stage. Artificial activation by cycloheximide, SrCl2 , and TPEN also reduced the amounts of zinc ions in the PN stage. On the other hand, PN embryos derived from sperm injection still showed high level of zinc ions. However, the amounts of zinc ions rapidly increased at the blastocysts regardless of activation method. We showed here that the amounts of zinc ions dramatically changed during maturation, fertilization, activation, and development in mouse oocytes. |
|
言語 |
en |
| 書誌情報 |
Animal Science Journal
巻 93,
号 1,
p. e13759-e13759,
発行日 2022-07-25
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1344-3941 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1111/asj.13759 |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
ASJ_final ver-Fig_supplement.pdf (5 MB)
