| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-10 |
| タイトル |
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|
タイトル |
Ganglioside GM3 deficiency enhances mast cell sensitivity |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Komuro, Mariko
Mizugaki, Hinano
Nagane, Masaki
Morimoto, Misako
Fukuyama, Tomoki
Ogihara, Kikumi
Naya, Yuko
Yokomori, Emi
Kaneshima, Kimika
Kawakami, Yasushi
Kamiie, Junichi
Shibata, Yuki
Suzuki, Mira
Shimizu, Takuto
Kawashima, Nagako
Okamoto, Mariko
Ikeda, Teruo
Yamashita, Tadashi
|
| Abstract |
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内容記述タイプ |
Abstract |
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内容記述 |
Mast cells are a significant source of cytokines and chemokines that play a role in pathological processes. Gangliosides, which are complex lipids with a sugar chain, are present in all eukaryotic cell membranes and comprise lipid rafts. Ganglioside GM3, the first ganglioside in the synthetic pathway, is a common precursor of the specifying following derivatives and is well known for its various functions in biosystems. Mast cells contain high levels of gangliosides; however, the involvement of GM3 in mast cell sensitivity is unclear. Therefore, in this study, we elucidated the role of ganglioside GM3 in mast cells and skin inflammation. GM3 synthase (GM3S)-deficient mast cells showed cytosolic granule topological changes and hyperactivation upon IgE-DNP stimulation without affecting proliferation and differentiation. Additionally, inflammatory cytokine levels increased in GM3S-deficient bone marrow-derived mast cells (BMMC). Furthermore, GM3S-KO mice and GM3S-KO BMMC transplantation showed increased skin allergic reactions. Besides mast cell hypersensitivity caused by GM3S deficiency, membrane integrity decreased and GM3 supplementation rescued this loss of membrane integrity. Additionally, GM3S deficiency increased the phosphorylation of p38 mitogen-activated protein kinase (MAPK). These results suggest that GM3 increases membrane integrity, leading to suppression of the p38 signaling pathway in BMMC and contributing to skin allergic reaction. |
|
言語 |
en |
| 書誌情報 |
The FEBS Journal
巻 290,
号 17,
p. 4268-4280,
発行日 2023-04-26
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| 出版者 |
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出版者 |
Wiley |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/febs.16806 |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
Clean GM3S_regulates_Skin_Allergy-12.pdf (3.2 MB)
