Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position

Noda, Kyoko; Hirakawa, Yuki; Nishino, Tomomi; Sekizuka, Ritsuto; Kishimoto, Marin; Furukawa, Tomohiro; Sawane, Sakiko; Matsunaga, Ayu; Kobayashi, Naoki; Sugita, Kazutoshi; Oonaka, Kenji; Kawakami, Hiroko; Otsuka, Yuji; Yamamoto, Tetsuya; Yamamoto, Toshihiro; Yoshiya, Taku; Watanabe, Maiko; Saka, Machiko; Momma, Keiko; Kushiro, Masayo; Miyake, Shiro

doi:10.3390/toxins14110747

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Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position

https://az.repo.nii.ac.jp/records/2000293

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toxins-14-00747.pdf (976 KB)

Item type

学術雑誌論文 / Journal Article(1)

公開日

2025-02-14

タイトル

Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position

言語

eng

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資源タイプ識別子

http://purl.org/coar/resource_type/c_6501

資源タイプ

journal article

著者

Noda, Kyoko
Hirakawa, Yuki
Nishino, Tomomi
Sekizuka, Ritsuto
Kishimoto, Marin
Furukawa, Tomohiro
Sawane, Sakiko
Matsunaga, Ayu
Kobayashi, Naoki
Sugita, Kazutoshi
Oonaka, Kenji
Kawakami, Hiroko
Otsuka, Yuji
Yamamoto, Tetsuya
Yamamoto, Toshihiro
Yoshiya, Taku
Watanabe, Maiko
Saka, Machiko
Momma, Keiko
Kushiro, Masayo
Miyake, Shiro

Abstract

内容記述タイプ

Abstract

内容記述

Nivalenol (NIV) is a trichothecene mycotoxin that is more toxic than deoxynivalenol. It accumulates in grains due to infection with Fusarium species, which are the causative agents of scab or Fusarium head blight. An immunoassay, which is a rapid and easy analytical method, is necessary for monitoring NIV in grains. However, a specific antibody against NIV has not been prepared previously. To establish an immunoassay, we prepared NIV, introduced a linker, and generated antibodies against it. NIV was prepared from a culture of Fusarium kyushuense obtained from pressed barley through chromatographic procedures with synthetic adsorbents and silica gel. NIV was reacted with glutaric anhydride, and the reaction was stopped before mono-hemiglutaryl-NIV was changed to di-hemiglutaryl-NIV. 15-O-Hemiglutaryl-NIV was isolated via preparative HPLC and bound to keyhole limpet hemocyanin (KLH) using the active ester method. Two different monoclonal antibodies were prepared by immunizing mice with the NIV-KLH conjugate. The 50% inhibitory concentration values were 36 and 37 ng/mL. These antibodies also showed high reactivity in a direct competitive enzyme-linked immunosorbent assay and specifically reacted with NIV and 15-acetyl-NIV but not with deoxynivalenol and 4-acetyl-NIV.

言語

書誌情報

Toxins

巻 14, 号 11, p. 1-13, 発行日 2022-10-31

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MDPI

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DOI

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2025-02-05 05:11:50.125371

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Preparation of Monoclonal Antibodies Specifically Reacting with the Trichothecene Mycotoxins Nivalenol and 15-Acetylnivalenol via the Introduction of a Linker Molecule into Its C-15 Position

× Noda, Kyoko

× Hirakawa, Yuki

× Nishino, Tomomi

× Sekizuka, Ritsuto

× Kishimoto, Marin

× Furukawa, Tomohiro

× Sawane, Sakiko

× Matsunaga, Ayu

× Kobayashi, Naoki

× Sugita, Kazutoshi

× Oonaka, Kenji

× Kawakami, Hiroko

× Otsuka, Yuji

× Yamamoto, Tetsuya

× Yamamoto, Toshihiro

× Yoshiya, Taku

× Watanabe, Maiko

× Saka, Machiko

× Momma, Keiko

× Kushiro, Masayo

× Miyake, Shiro

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