| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-24 |
| タイトル |
|
|
タイトル |
Aberrant histone methylation in mouse early preimplantation embryos derived from round spermatid injection |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Ooga, Masatoshi
Kikuchi, Yasuyuki
Ito, Daiyu
Kazama, Kousuke
Inoue, Rei
Sakamoto, Mizuki
Wakayama, Sayaka
Wakayama, Teruhiko
|
| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
Round spermatid injection (ROSI) is the last resort and recourse for men with nonobstructive azoospermia to become biological fathers of their children. However, the ROSI-derived offspring rate is lower than intracytoplasmic sperm injection (ICSI) in mice (20% vs. 60%). This low success rate has hindered the spread of ROSI in ART (Assisted Reproductive Technology). However, the cause of the ROSI-zygote-derived low offspring rate is currently unknown. In the previous studies, we reported that H3K9me3 and H3K27me3 exhibited ectopic localizations in male pronuclei (mPN) of ROSI-zygotes, suggesting that the carried over histone to zygotes conveys epigenetic information. In this study, we analyzed other histone modifications to explore unknown abnormalities. H3K36me3 showed an increased methylation state compared to ICSI-derived embryos but not for H3K4me3. Abnormal H3K36me3 was corrected until 2-cell stage embryos, suggesting a long window of reprogramming ability in ROSI-embryos. Treatment with TSA of ROSI-zygotes, which was reported to be capable of correcting ectopic DNA methylation in ROSI-zygotes, caused abnormalities of H3K36me3 in male and female PN (fPN) of the zygotes. In contrast, round spermatid TSA treatment before ROSI, which was reported to improve the preimplantation development of ROSI-zygotes, showed beneficial effects without toxicity in fPN. Therefore, the results suggest that TSA has some negative effects, but overall, it is effective in the correction of epigenetic abnormalities in ROSI-zygotes. When attempting to correct epigenetic abnormalities, attention should be paid to epigenomes not only in male but also in female pronuclei. |
|
言語 |
en |
| 書誌情報 |
Biochemical and Biophysical Research Communications
巻 680,
p. 119-126,
発行日 2023-09-12
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| 出版者 |
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出版者 |
Elsevier BV |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0006-291X |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1016/j.bbrc.2023.09.020 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
Aberrant histone methylation in mouse early preimplantation embryos derived from round spermatid injection.pdf (4.5 MB)
