| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-15 |
| タイトル |
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|
タイトル |
A thioacetamide-induced liver fibrosis model for pre-clinical studies in microminipig |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Nishi, Kotaro
Yagi, Hiroshi
Ohtomo, Mana
Nagata, Shogo
Udagawa, Daisuke
Tsuchida, Tomonori
Morisaku, Toshinori
Kitagawa, Yuko
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| Abstract |
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内容記述タイプ |
Abstract |
|
内容記述 |
Drug-induced liver fibrosis models are used in normal and immunosuppressed small animals for transplantation and regenerative medicine to improve liver fibrosis. Although large animal models are needed for pre-clinical studies, they are yet to be established owing to drug sensitivity in animal species and difficulty in setting doses. In this study, we evaluated liver fibrosis by administering thioacetamide (TA) to normal microminipig and thymectomized microminipig; 3 times for 1 week (total duration: 8 weeks). The pigs treated with TA showed elevated blood cytokine levels and a continuous liver injury at 8 weeks. RNA-seq of the liver showed increased expression of fibrosis-related genes after TA treatment. Histopathological examination showed degenerative necrosis of hepatocytes around the central vein, and revealed fibrogenesis and hepatocyte proliferation. TA treatment caused CD3-positive T cells and macrophages scattered within the hepatic lobule to congregate near the center of the lobule and increased αSMA-positive cells. Thymectomized pigs showed liver fibrosis similar to that of normal pigs, although the clinical signs tended to be milder. This model is similar to pathogenesis of liver fibrosis reported in other animal models. Therefore, it is expected to contribute to research as a drug discovery and pre-clinical transplantation models. |
|
言語 |
en |
| 書誌情報 |
Scientific Reports
巻 13,
号 1,
p. 14996-14996,
発行日 2023-09-11
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| 出版者 |
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|
出版者 |
Springer Nature |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1038/s41598-023-42144-8 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 査読 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
査読あり |
s41598-023-42144-8.pdf (9.7 MB)
