@article{oai:az.repo.nii.ac.jp:00005169,
 author = {猪股, 智夫 and Inomata, Tomo},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {ラットにgpt遺伝子を導入できたが,gpt遺伝子を含むλEG10DNAは確認できなかった。, The evaluation of chemicals which induce mutations is a particularly important issue in the field of environmental mutagenesis and carcinogenesis. Transgenic mouse (gpt delta, mouse) have been produced by introducing lEG10 phage DNA, composed of the Escherichia gpt gene and red/gam gene of lphage, because they are thought to be useful in effectively evaluating the safety of new chemicals in vivo. However, the sample size taken from the mouse is limited. It is necessary to produce transgenic rats, which have the same characteristics of gpt delta mice. We produced 11 transgenic rats as a founder having gpt gene. In the next generation, 56 pups out of a total of 125 also had gpt gene. We examined a collection efficiency of lEG10DNA including gpt gene form their DNA in vitro. 56 rats having gpt gene were used. A small part of liver of in each rat was removed under anesthesia. DNA was extracted form the liver. The DNA collection efficiency of lEG10DNA including gpt gene was examined by in vitro packaging method. As for each rat, it was confirmed that gpt gene was introduced. However, lEG10DNA was collected in neither individual by the packaging method. It may be thought that a little number of the copies of lEG10DNA incorporated into genomic DNA, or that a body portion of 1 phage being broken even if lEG10DNA was introduced., 麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ3, P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {307--308},
 title = {トランスジェニックマウスを用いたコプラナーPCBsの毒性評価 : 化学物質の安全性評価モデルTgラットの開発},
 volume = {13/14},
 year = {2007},
 yomi = {イノマタ, トモオ}
}