{"created":"2023-06-19T07:19:13.781554+00:00","id":5168,"links":{},"metadata":{"_buckets":{"deposit":"80dc9246-027a-4331-a059-49ab889aa630"},"_deposit":{"created_by":4,"id":"5168","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5168"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005168","sets":["17:253:315","31:181:511"]},"author_link":["22493","22494"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"306","bibliographicPageStart":"305","bibliographicVolumeNumber":"13/14","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Atlg17180およびAtlg78340のGST遺伝子の内,レポーター遺伝子GUS,GFPを有するAtlg17180遺伝子プロモーター領域のタバコ培養細胞BY-2ゲノムへの導入に成功した。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Dioxins such as PCDDs, PCDFs and dioxin-like coplanar PCBs (Co-PCBs) are hard to be decomposed due to their stability and hydrophobic nature, leading to the world-wide contamination. The precise quantitative analysis of pollution levels of dioxins has been performed using a gas chromatograph equipped with the high-resolution mass spectrometry; however, this technique has the disadvantage of a high cost or a highly educated skill. In recent years it has become evident that the expression of several genes in animals was changed in response to dioxins treatment, and then this makes these genes potential candidates for use as the biomarker of exposure to dioxins. This biomarker-monitoring system will be expected to be a good substitution for the instrumental analysis as the first step analysis of dioxins in the environment. Until now, several dioxins-response genes in the genome of Arabidopsis thaliana (A. thaliana) had been found. Among them, cytochrome P450 monooxygenases, glutathione S-transferases and peroxidases which were involved in the xenobiotic transformation were found to be up-regulated by the exposure to dioxins. Especially, glutathione S-transferases genes, Atlg17180 and Atlg78340, had the high response specificity to the exposure time and exposure chemicals. In this study, the cloning of promoter region of Atlg17180 and Atlg78340 with the promoter-analysing vector pBGWFS7.0 and the introduction of promoter region into the genome of Nicotiana tabacum BY-2(cultured tobacco cell) were tried. As a result the promoter region of Atlg17180 was successfully introduced into the tobacco cell, and the introduction was confirmed with PCR.","subitem_description_type":"Other"}]},"item_12_description_18":{"attribute_name":"レポート・演題番号","attribute_value_mlt":[{"subitem_description":"麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ2","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000120"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学環境保健学部"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"School of Environmental Health, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000120"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"其木, 茂則"},{"creatorName":"ソノキ, シゲノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sonoki, Shigenori","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2006-01-01"}],"displaytype":"detail","filename":"bull_jau_vol13-14-305.pdf","filesize":[{"value":"112.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol13-14-305.pdf","url":"https://az.repo.nii.ac.jp/record/5168/files/bull_jau_vol13-14-305.pdf"},"version_id":"d71a0471-5953-40d6-86db-29fae9921e8d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"形質転換植物を用いたダイオキシン類による汚染環境のバイオモニタリングに関する基礎研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"形質転換植物を用いたダイオキシン類による汚染環境のバイオモニタリングに関する基礎研究"},{"subitem_title":"Studies on biomonitoring of dioxins in the polluted environment using transgenic plants.","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["315","511"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-01-01"},"publish_date":"2006-01-01","publish_status":"0","recid":"5168","relation_version_is_last":true,"title":["形質転換植物を用いたダイオキシン類による汚染環境のバイオモニタリングに関する基礎研究"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:48:45.077462+00:00"}