{"created":"2023-06-19T07:19:13.613243+00:00","id":5164,"links":{},"metadata":{"_buckets":{"deposit":"48813c2f-5390-402a-89ed-d932b0f29133"},"_deposit":{"created_by":4,"id":"5164","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5164"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005164","sets":["17:236:231","17:253:312","17:253:315","17:253:325","31:181:511"]},"author_link":["22467","22458","22462","22464","22463","22465","22466","22457","22461","22460","22468","22459"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"291","bibliographicPageStart":"287","bibliographicVolumeNumber":"13/14","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"化学物質暴露下の生体影響を血漿タンパク質およびゲノム解析より評価した。第一に表現系である血漿等タンパク質分析には迅速ミクロ2次元電気泳動?を駆使したが,特にメダカの血漿分析では1個体からの分析がはじめて可能になった。この系を用いた化学物質,BPA (bisphenolA)の毒性評価への適用を試みた結果,雄メダカ1個体中の血漿中にビテロジェニンが用量依存的に検出できることを確認した。加えて,ムラサキイガイおよびラットについても同様の系を用いると,ムラサキイガイの体液を用いた2次元電気泳動分析ではアルカリ側に複数のスポットが確認できた。現在,BPA暴露による影響を解析中である。また,ラットではDES(diethylstilbestrol)投与により雄個体の精子数の減少傾向とα2u-グロブリンの産生時期が遅延することが確認できた。第二のゲノム解析には暴露下の植物遺伝子発現等の遺伝子解析を行い次の結果が得られた。cDNAマイクロアレイ,およびリアルタイムRT-PCR法を用いて,PCB126(3, 3', 4, 4', 5-pentachlorobiphenyl)とTCDD(2, 3, 7, 8-tetrachlorodibezo-p-dioxin)暴露により発現が促進する2種類のグルタチオンS-トランスフェラーゼ遺伝子(GST)をシロイヌナズナゲノム中に見出した。これらの成績により植物,水棲生物およびラット等を用いた化学物質の網羅的な影響評価の可能性が示唆され,プロテオーム解析につながる生体影響評価システム構築の一部を達成することができた。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The biological effects of exposure to chemicals were evaluated using plasma protein and genomic analyses. Firstly, a high-speed micro 2-dimensional electrophoresis was used for plasma protein analysis, which is an expression system. With particular regard to plasma analysis for Medaka (Oryzias latipes), analysis on an individual basis became possible for the first time. Application of this individualized analysis system to toxicity assessment for bisphenol A (BPA) revealed that vitellogenin was present dose-dependently in the plasma of one male Medaka. In addition, toxicity assessments using a similar system were carried out in marine invertebrates and rats. In 2-dimensional electrophoresis analysis using the body fluid of blue mussel(Mytilus edulis), multiple spots were confirmed on the alkaline side. The effects of BPA exposure are currently being analyzed. In addition, in rats, administration of diethylstilbestrol (DES) resulted in delayed production of α2u-globulin in male individuals. Secondly, for genomic analysis, gene analysis of factors such as gene expression in plants under exposure was conducted. Subsequently, two types of glutathione S-transferase (GST) genes whose expression are enhanced upon exposure to PCB126 (3, 3', 4, 4', 5-pentachlorobiphenyl) and TCDD (2, 3, 7, 8-tetrachlorodibezo-p-dioxin) were identified in the genome of Arabidopsis using cDNA microarray and real-time RT-PCR. These findings suggest the feasibility of comprehensive impact assessments for exposure to chemicals in organisms such as plants, aquatic organisms, and rats, and contributed to establishment of a biological effect assessment system in addition to proteome analysis.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE 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UNIV"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"000120"},{"subitem_text_value":"000088"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 潤"},{"creatorName":"スズキ, ジュン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"其木, 茂則"},{"creatorName":"ソノキ, シゲノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐俣, 哲郎"},{"creatorName":"サマタ, テツロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"本田, 政幸"},{"creatorName":"ホンダ, マサユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"宮川, 洋三"},{"creatorName":"ミヤカワ, ヨウゾウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"河村, 好章"},{"creatorName":"カワムラ, ヨシアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Jun","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sonoki, Shigenori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Samata, Teturou","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Honda, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyakawa, Youzou","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawamura, Yoshiaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2006-01-01"}],"displaytype":"detail","filename":"bull_jau_vol13-14-287.pdf","filesize":[{"value":"497.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol13-14-287.pdf","url":"https://az.repo.nii.ac.jp/record/5164/files/bull_jau_vol13-14-287.pdf"},"version_id":"a8fcf837-1294-47bf-a033-08cbda03dca7"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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