{"created":"2023-06-19T07:19:13.274009+00:00","id":5156,"links":{},"metadata":{"_buckets":{"deposit":"85ed384a-3217-45a1-9959-83658b882156"},"_deposit":{"created_by":4,"id":"5156","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5156"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005156","sets":["17:232:208","17:38:262","31:181:511"]},"author_link":["22407","22409","22410","22412","22411","22408"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"256","bibliographicPageStart":"253","bibliographicVolumeNumber":"13/14","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"近年, PCR-RFLP法は, 形態学的に鑑別不可能なAnisakis simplex同胞種(A.simplex sensu stricto, A.pegreffii, A.simplex C)の同定法として,その有効性が証明された。そこで本研究では,日本近海に生息し,形態学的にA. simplexと同定された虫体について,rRNA遺伝子ITS領域のPCR-RFLP解析を行った。その結果,ITS領域の制限酵素切断パターンにより,北方域の幼虫(100%)と成虫(94%)はA. simplex s.str.,南方域の幼虫(97%)はA.pegreffiiと同定した。又,今回は,これら同胞種2種の混合感染は認められなかったが,両地域において交雑型を検出した。さらに,アニサキス症患者から摘出された虫体についても,同法により同定を行ったところ,調査した患者の99%より摘出された虫体はA.simplex s.str.であり,九州の患者1名(1%)のみでA.pegYeffiiの感染が認められた。日本近海ではA.simplex s.str.とA.pegreffiの2種が生息し,南北に分かれて分布しているが,アニサキス症においてはA.simplex s.str.が最も重要な病原虫と示唆された。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Recently, new markers based on PCR-RFLP analysis was proved to be useful for the identification of the sibling species of Anisakis simplex, i.e. A. simplex sensu stricto, A. pegreffii and A. simplex C. In this study, parasites morphologically consistent with Anisakis simplex collected from the coast of Japan and Western North Pacific Ocean were examined by PCR-RFLP of the ITS region (ITS1, 5.8 subunit rRNA gene and ITS2). The RFLP patterns generated by HinfI and HhaI showed that 100% of the larvae collected from Hokkaido and 94% of adults collected from Western North Pacific Ocean were identified as A.simplex s.str. On the other hand, 97% of the larvae collected from Fukuoka prefecture were identified as A. pegreffii. A hybrid genotype was found in adults in Western North Pacific Ocean and larva in Fukuoka prefecture. One hundred larvae from eighty five patients with Anisakiasis in Hokkaido and Kyusyu were also used for the molecular identification. The RFLP patterns showed that five larvae in Hokkaido and ninety four larvae in Kyusyu were identified as A.simplex s.str., only one larva that infected male patient in Kyusyu was identified as A.pegreffii. We found that although A.simplex s.str. is primarily distributed in the North Pacific Ocean and A.pegreffii is primarily distributed in the southern Sea of Japan, A.simplex s.str. is the most important etiological agent in Japan.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"000130"},{"subitem_text_value":"000121"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院環境保健学研究科環境保健科学専攻"},{"subitem_text_value":"麻布大学大学院環境保健学研究科環境保健科学専攻"},{"subitem_text_value":"麻布大学大学院環境保健学研究科環境保健科学専攻"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Course of Environmental Health Science, Azabu University"},{"subitem_text_value":"Course of Environmental Health Science, Azabu University"},{"subitem_text_value":"Course of Environmental Health Science, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"000130"},{"subitem_text_value":"000121"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"内田, 明彦"},{"creatorName":"ウチダ, アキヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"川上, 泰"},{"creatorName":"カワカミ, ヤスシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"堂ヶ崎, 知格"},{"creatorName":"ドウガサキ, チカク","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uchida, Akihiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawakami, Yasushi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dogasaki, Chikaku","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2006-01-01"}],"displaytype":"detail","filename":"bull_jau_vol13-14-253.pdf","filesize":[{"value":"421.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol13-14-253.pdf","url":"https://az.repo.nii.ac.jp/record/5156/files/bull_jau_vol13-14-253.pdf"},"version_id":"37c8a62e-3ea7-47e7-9963-da4c3529361b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アニサキス属線虫の遺伝子変異と抗原多型を解明する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アニサキス属線虫の遺伝子変異と抗原多型を解明する研究"},{"subitem_title":"Genetic polymorphism and antigen diversity of parasitic nematodes of genus Anisakis","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["208","262","511"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-01-01"},"publish_date":"2006-01-01","publish_status":"0","recid":"5156","relation_version_is_last":true,"title":["アニサキス属線虫の遺伝子変異と抗原多型を解明する研究"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:50:33.854831+00:00"}