{"created":"2023-06-19T07:19:13.185487+00:00","id":5154,"links":{},"metadata":{"_buckets":{"deposit":"08ccc44c-3667-469a-83c3-8536c1c04a0b"},"_deposit":{"created_by":4,"id":"5154","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5154"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005154","sets":["17:234:212","17:234:238","17:251:300","31:181:511"]},"author_link":["22400","22398","22397","22395","22396","22399"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"247","bibliographicPageStart":"241","bibliographicVolumeNumber":"13/14","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vibrio vulnificus感染症の感染経路や感染源を解明する一環として,環境由来株とヒト臨床由来株について血清型別,各種抗菌剤の薬剤感受性試験およびPFGE法によりDNA解析を行い分子疫学的検討を行ったところ,以下の成績が得られた。1.由来別に血清型を検討したところ,環境由来では72.5%が18菌型に型別され,O7が43.1%と最も多く,次にO4が6.1%などであった。ヒト臨床由来では87.1%が8菌型に型別され,O4が43.5%と最も多く,次にO7が12.9%などであった。その地域別において,東日本地域では69.2%が18菌型に型別され,O7が44.6%,04が5.7%などに,西日本地域では64.8%が8菌型に型別され,O7が20.4%と最も多く,次にO4が11.1%などにそれぞれ型別された。2.由来別に薬剤感受性をMIC_<90>で比較検討したところ,環境由来ではABPC,PIPC,CPZ,CTX,LMOX,MEPM,GM,EM,TC,DOXY,MINO,CP,NAおよびCPFXに感受性を示したが,CER,CET,CTX,CMZ,KMおよびLCMに対して耐性株が認められた。ヒト臨床由来ではEM,TC,DOXY,MINO,CP,NAおよびCPFXに感受性を示したが,ABPC,PIPC,CER,CET,CPZ,CTX,CMZ,LMOX,MEPM,KM,GM,AMKおよびLCMに対して耐性株が認められた。3.Not IとSfi Iの2種類の酵素を用いてそれぞれDNA切断を行い,PFGE法でDNA解析を検討したところ,酵素別のDNAパターン判読率は,Not I では76.9%,sfi Iでは97.9%を示し本菌のDNAパターン判読率はSfi Iが優れていた。4.Sfi IによってDNAパターンが判読された菌株をUPGMA法で解析を行ったところ,類似度が低く多岐のクラスターを示し,本菌感染症は複数のクローンから発生していることが明らかとなった。また,由来等が異なるが類似度が89%以上の類似度で,臨床株と環境株の組合せが認められることから,環境からヒト,ヒトから環境への感染の可能性が考えられた。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"As a part of elucidation of the route and source of Vibrio vulnificus infection, antimicrobial drug sensitivity tests and molecular-epidemiological investigation by DNA analysis using PFGE of each serotype of environmental and human clinical strains were performed, and the following findings were obtained. 1. On serotyping of the strains from each source, 72.5% of the environmental strains belonged to 18 types : the most frequent type was O7, accounting for 43.1%, followed by O4 accounting for 6.1%. As for the human clinical strains, 87.1% belonged to 8 types : the most frequent type was O4, accounting for 43.5%, followed by O7 accounting for 12.9%. 3. On determination of MIC_<90> for the strains from each source as drug sensitivity, the environmental strains were sensitive to ABPC, PIPC, CPZ, CTX, LMOX, MEPM, GM, EM, TC, DOXY, MINO, CP, NA, and CPFX, but there were strains resistant to CER, CET, CTX, CMZ, KM, and LCM. The human clinical strains were sensitive to EM, TC, DOXY, MINO, CP, NA, and CPFX, but there were strains resistant to ABPC, PIPC, CER, CET, CPZ, CTX, CMZ, LMOX, MEPM, KM, GM, AMK, and LCM. 4. DNA was cleaved with 2 enzymes, Not I and Sfi I, and DNA was analyzed by PFGE. The DNA pattern reading rate was 76.9% on cleavage with Not I and 97.9% with Sfi I, showing that Sfi I was superior in reading the DNA patterns. 5. When strains whose DNA patterns were read on cleavage with Sfi I were analyzed by the UPGMA method, the similarity was low, and various clusters were present, clarifying that this infectious disease is caused by multiple clones. Moreover, combinations of clinical and environmental strains with 89% or higher similarity were noted, although the sources were different, showing a possibility of transmission from the environment to humans and vice versa.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000249"},{"subitem_text_value":"000279"},{"subitem_text_value":"000119 "}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学環境保健学部"},{"subitem_text_value":"麻布大学大学院環境保健学研究科"},{"subitem_text_value":"麻布大学大学院環境保健学研究科"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"College of Environmental Health, Azabu University"},{"subitem_text_value":"GraduateSchool of Environmental Health Sciences, Azabu University"},{"subitem_text_value":"GraduateSchool of Environmental Health Sciences, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000249"},{"subitem_text_value":"000279"},{"subitem_text_value":"000119 "}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大仲, 賢二"},{"creatorName":"オオナカ, ケンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"古畑, 勝則"},{"creatorName":"フルハタ, カツノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"福山, 正文"},{"creatorName":"フクヤマ, マサフミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oonaka, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Furuhata, Katunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuyama, Masafumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2006-01-01"}],"displaytype":"detail","filename":"bull_jau_vol13-14-241.pdf","filesize":[{"value":"632.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol13-14-241.pdf","url":"https://az.repo.nii.ac.jp/record/5154/files/bull_jau_vol13-14-241.pdf"},"version_id":"9993e76f-eab4-452f-a060-7938b6939aba"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Vibrio vulnificus感染症に関する疫学的検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Vibrio vulnificus感染症に関する疫学的検討"},{"subitem_title":"Epidemiological study of Vibrio vulnificus infection","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["212","238","300","511"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-01-01"},"publish_date":"2006-01-01","publish_status":"0","recid":"5154","relation_version_is_last":true,"title":["Vibrio vulnificus感染症に関する疫学的検討"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:49:25.114046+00:00"}