{"created":"2023-06-19T07:19:12.241742+00:00","id":5132,"links":{},"metadata":{"_buckets":{"deposit":"70db2c65-9fa7-48f4-abd9-8bfa8ad711d8"},"_deposit":{"created_by":4,"id":"5132","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5132"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005132","sets":["17:254:337","17:254:339","17:38:266","31:181:511"]},"author_link":["22271","22269","22266","22268","22270","22267"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"143","bibliographicPageStart":"141","bibliographicVolumeNumber":"13/14","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"妊娠の維持と調節機構にNOが重要な役割を有していることが示唆されているが,NOは不安定なフリーラジカルであるため,解析が困難であった。近年,ジチオカルバメート鉄錯体であるFe-DTCS(Fe-N-(dithiocarboxy)-sarcosine)を用いて,不安定なNOを安定なNO-Fe-DTCS錯体にトラップした後,電子常磁性共鳴吸収(electron paramagnetic resonance: EPR)装置により解析することにより,NO産生を検出し,定量化できることが報告されている。本研究では,このスピントラップ-EPR法により妊娠ラットを用いて,妊娠中期に発現がピークを示す胎盤におけるNO産生の役割を検討した。NOS阻害剤L-NAMEを用いて,一時的にNO産生を抑制したモデルを作製したところ,NO産生の低下とともに血管新生の主要因子であるVEGFの発現が抑制されたが,その後徐々に回復した。この結果は,胎盤において産生されているNOはVEGFの発現を誘導している可能性を示唆している。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The NO production level was examined by electron paramagnetic resonance (EPR) spectroscopy with Fe-N- (dithiocarboxy) sarcosine (DTCS) complex as NO-trapping reagent. The expression of nitric oxide synthase (NOS) isoform was also examined by quantitative reverse transcriptase-mediated polymerase chain reaction (RT-PCR). In the placenta, NO production was first detected by EPR spectroscopy on day 13.5 of gestation, and the NO level reached a peak on day 15.5 of gestation, and then significantly decreased through the last few days of gestation. The expression of NOS II mRNA as measured by quantitative RT-PCR was stronger than that of NOS III mRNA, and the NOS II mRNA expression pattern was in good agreement with the NO production pattern, whereas NOS III mRNA expression showed no marked changes during gestation. In addition, the VEGF mRNA expression in the placenta was examined to clarify the interaction of NO and VEGF in the placenta using continues L-NAME, NOS inhibitor, infusion model. The VEGF mRNA expression was significant decreased through the L-NAME treatment. The present results indicate that placental NO production was gestational stage-dependent, and the peak of NO production on day 15.5 was mainly regulated by the expression of NOS II. The expression of VEGF was reduced by L-NAME treatment, suggesting that NO stimulate the VEGF expression in the mid-term placenta.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000244"},{"subitem_text_value":"000316"},{"subitem_text_value":"000334"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院獣医学研究科"},{"subitem_text_value":"麻布大学大学院獣医学研究科"},{"subitem_text_value":"麻布大学大学院獣医学研究科"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Graduate School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Graduate School of Veterinary Medicine, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000244"},{"subitem_text_value":"000316"},{"subitem_text_value":"000334"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"滝沢, 達也"},{"creatorName":"タキザワ, タツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"神作, 宜男"},{"creatorName":"カンサク, ノリオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田中, 和明"},{"creatorName":"タナカ, カズアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takizawa, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kansaku, Norio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Kazuaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2006-01-01"}],"displaytype":"detail","filename":"bull_jau_vol13-14-141.pdf","filesize":[{"value":"274.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol13-14-141.pdf","url":"https://az.repo.nii.ac.jp/record/5132/files/bull_jau_vol13-14-141.pdf"},"version_id":"59019614-f24e-4440-93d3-764863e2333c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"胎盤と子宮などにおけるNO産生の意義とNOS遺伝子発現調節機構 : 妊娠中期胎盤におけるNOの役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胎盤と子宮などにおけるNO産生の意義とNOS遺伝子発現調節機構 : 妊娠中期胎盤におけるNOの役割"},{"subitem_title":"Expression of nitric oxide syntheses isforms and detection of nitric oxide in rat placenta during pregnancy : Possible role of NO on vasculogenesis in rat placenta","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["266","337","339","511"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-01-01"},"publish_date":"2006-01-01","publish_status":"0","recid":"5132","relation_version_is_last":true,"title":["胎盤と子宮などにおけるNO産生の意義とNOS遺伝子発現調節機構 : 妊娠中期胎盤におけるNOの役割"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:48:21.374424+00:00"}