@article{oai:az.repo.nii.ac.jp:00005131,
 author = {須永, 藤子 and 斉藤, 康秀 and 並河, 和彦 and Sunaga, F. and Saito, Y. and Namikawa, K.},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {B.gibsoni原虫の培養上清中に存在する抗原物質が有望なワクチン候補であることをすでに報告したが,培養上清中には多数の抗原物質が含まれており,有効な防御抗原物質は特定されていない。そこで,防御抗原を特定する目的で,原虫培養上清をイオン交換クロマトグラフィーで2つの成分に分け,それぞれの抗原の防御免疫効果を検討した。その結果,両免疫群ともに対照群に比べ,原虫の増殖,貧血および発熱が抑制されたが,F1免疫群の貧血抑制効果はF2免疫群に比べ優れていた。これらの結果からF1成分中により有効なワクチン候補となる抗原が存在することが示唆された。, The antigen was made by concentrating the supernatant of B.gibsoni culture medium with the ultrafilter, and was divided into F1 and F2 by ion exchange chromatography. These antigens were subcutaneously injected to two female one-year old beagles respectively, and were examined for immunogenicity by the indirect fluorescent method for 60 days after the inoculation. The antibody showed a titer of 1, 280 all four in dogs. Then, two groups of dogs inoculated with F1 and F2 antigens respectively were challenged with the virulent B.gibsoni isolate, and F1 antigen group compared with F2 antigen group in the preventive activity of the attack with such indexes as the preventive rate of the multiplication of parasites, anemia and pyrexia. Consequently, the suppressive effects of the multiplication of parasites and pyrexia were recognized in both groups, especially the suppressive effect of anemia was excellently observed in the F1 antigen group. These results suggested that the F1 antigenic substance in the supernatant of culture medium had an effective activity as inactivated vaccine., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {138--140},
 title = {Babesia gibsoni症のワクチン開発},
 volume = {13/14},
 year = {2007},
 yomi = {スナガ, フジコ and サイトウ, ヤスヒデ and ナミカワ, カズヒコ}
}