{"created":"2023-06-19T07:19:09.523431+00:00","id":5069,"links":{},"metadata":{"_buckets":{"deposit":"992a9be3-d9a0-4fcf-aeb9-6c662d82a5bd"},"_deposit":{"created_by":4,"id":"5069","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5069"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005069","sets":["17:253:317","17:253:318","17:40:280","31:181:494"]},"author_link":["22018","22021","22022","22020","22023","22015","22017","22016","22014","22019"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"290","bibliographicPageStart":"286","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"これまでの実験で,PCB126暴露により次世代動物の卵巣における発育卵胞数の減少,E_2産生の低下と共に,卵巣におけるArom遺伝子発現量の減少が認められた。そこで,本実験では妊娠15日にPCB126(50μg/kg)暴露を受けた出生雌ラットの卵巣内発育卵胞を,発育段階により形態学的に分類してその数を数え,影響の認められた発育段階の卵胞からLMD法により類粒層細胞を採取して遺伝子発現量の変化を検索した。その結果,暴露動物では15日齢において卵胞腔形成期の卵胞が減少しており,これら発育段階にある卵胞の顆粒層細胞ではAromとERβの遺伝子発現量が低下していた。いっぽう,顆粒層細胞のFSHRやIGF-1,あるいは卵細胞のGDF-9とBMP-15の遺伝子発現量には影響が認められなかった。これらの結果から,PCB126の卵胞発育の阻害は卵巣におけるestrogen作用の低下が関与していることが示唆された。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Estrogen actions are involved in the ovarian follicle development, and then this study aimed to examine whether the estrogen actions were involved in the modulation of follicle development induced by PCB126. Pregnant Sprague-Dawley rats were orally administrated the PCB126 at dose levels of 0 (corn oil), 50 or 100 μg/kg on gestational day (GD) 15. The ovaries were collected on postnatal days (PNDs) 10 and 15 from the offspring, and growing follicle numbers of primary and secondary in each ovary were counted, respectively. Furthermore, granulosa cells in the primary (with 50 μm～150 μm diameter) and secondary (with 151 μmi～250 μm diameter) follicles were then dissected by the Laser Microdissection (LMD), and mRNA expressions quantified by real-time PCR were compared between control and 50 μg/kg PCB126 group. The number of secondary follicles in the 50 and 100 μg/kg-exposed groups were decreased compared with that in age-matched control on PNDs 10 and 15 respectively, although that of primary follicles did not be altered at PND 15. The P450aromtase mRNA expressions in the granulosa cells isolated from both primary and secondary follicles were decreased in the group treated with 50 μg/kg PCB126. ERβ mRNA expression in the granulosa cells isolated from secondary follicles was also decreased by the PCB126 exposure at PND 15. In summary, the present study suggested the possibility that in utero and lactational exposure to PCB126 altered intra follicular E_2 synthesis by reducing the P450aromatase and ERβ mRNA expressions, and then reduced estrogen actions affected on primary to secondary follicle transition.","subitem_description_type":"Other"}]},"item_12_description_18":{"attribute_name":"レポート・演題番号","attribute_value_mlt":[{"subitem_description":"麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ1","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561557","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000049"},{"subitem_text_value":"-"},{"subitem_text_value":"000259"},{"subitem_text_value":"-"},{"subitem_text_value":"000406"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学生物科学総合研究所"},{"subitem_text_value":"麻布大学大学院獣医学研究科動物応用科学専攻"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"財・食品薬品安全センター秦野研究所"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Research Institute of Biosciences, Azabu University"},{"subitem_text_value":"Research Institute of Biosciences, Azabu University"},{"subitem_text_value":"School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"School of Veterinary Medicine, Azabu University /"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000049"},{"subitem_text_value":"-"},{"subitem_text_value":"000259"},{"subitem_text_value":"-"},{"subitem_text_value":"000406"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"代田, 欣二"},{"creatorName":"シロタ, キンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"櫻田, 陽右"},{"creatorName":"サクラダ, ヨウスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 賢"},{"creatorName":"ムラカミ, マサル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"赤堀, 文昭"},{"creatorName":"アカホリ, フミアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"代田, 眞理子"},{"creatorName":"シロタ, マリコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirota, Kinji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurada, Yosuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murakami, Masaru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Akahori, Humiaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shirota, Mariko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-286.pdf","filesize":[{"value":"432.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-286.pdf","url":"https://az.repo.nii.ac.jp/record/5069/files/bull_jau_vol15-16-286.pdf"},"version_id":"acb8d5d1-d81b-4dee-a265-f7444775965c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Co-PCBsの次世代に及ぼす影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Co-PCBsの次世代に及ぼす影響"},{"subitem_title":"The Effects of Exposure to Coplanar PCBs on the Progeny(Research Group 1,Azabu University High-Tech Research Center Project : Study on the Biological Toxicity and Development of the Technology for the Environmental Monitoring and Remediation of Dioxins and -Related Compounds)","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["280","317","318","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"5069","relation_version_is_last":true,"title":["Co-PCBsの次世代に及ぼす影響"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:43:56.660017+00:00"}