@article{oai:az.repo.nii.ac.jp:00005069,
 author = {代田, 欣二 and 櫻田, 陽右 and 村上, 賢 and 赤堀, 文昭 and 代田, 眞理子 and Shirota, Kinji and Sakurada, Yosuke and Murakami, Masaru and Akahori, Humiaki and Shirota, Mariko},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {これまでの実験で,PCB126暴露により次世代動物の卵巣における発育卵胞数の減少,E_2産生の低下と共に,卵巣におけるArom遺伝子発現量の減少が認められた。そこで,本実験では妊娠15日にPCB126(50μg/kg)暴露を受けた出生雌ラットの卵巣内発育卵胞を,発育段階により形態学的に分類してその数を数え,影響の認められた発育段階の卵胞からLMD法により類粒層細胞を採取して遺伝子発現量の変化を検索した。その結果,暴露動物では15日齢において卵胞腔形成期の卵胞が減少しており,これら発育段階にある卵胞の顆粒層細胞ではAromとERβの遺伝子発現量が低下していた。いっぽう,顆粒層細胞のFSHRやIGF-1,あるいは卵細胞のGDF-9とBMP-15の遺伝子発現量には影響が認められなかった。これらの結果から,PCB126の卵胞発育の阻害は卵巣におけるestrogen作用の低下が関与していることが示唆された。, Estrogen actions are involved in the ovarian follicle development, and then this study aimed to examine whether the estrogen actions were involved in the modulation of follicle development induced by PCB126. Pregnant Sprague-Dawley rats were orally administrated the PCB126 at dose levels of 0 (corn oil), 50 or 100 μg/kg on gestational day (GD) 15. The ovaries were collected on postnatal days (PNDs) 10 and 15 from the offspring, and growing follicle numbers of primary and secondary in each ovary were counted, respectively. Furthermore, granulosa cells in the primary (with 50 μm～150 μm diameter) and secondary (with 151 μmi～250 μm diameter) follicles were then dissected by the Laser Microdissection (LMD), and mRNA expressions quantified by real-time PCR were compared between control and 50 μg/kg PCB126 group. The number of secondary follicles in the 50 and 100 μg/kg-exposed groups were decreased compared with that in age-matched control on PNDs 10 and 15 respectively, although that of primary follicles did not be altered at PND 15. The P450aromtase mRNA expressions in the granulosa cells isolated from both primary and secondary follicles were decreased in the group treated with 50 μg/kg PCB126. ERβ mRNA expression in the granulosa cells isolated from secondary follicles was also decreased by the PCB126 exposure at PND 15. In summary, the present study suggested the possibility that in utero and lactational exposure to PCB126 altered intra follicular E_2 synthesis by reducing the P450aromatase and ERβ mRNA expressions, and then reduced estrogen actions affected on primary to secondary follicle transition., 麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ1, P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {286--290},
 title = {Co-PCBsの次世代に及ぼす影響},
 volume = {15/16},
 year = {2008},
 yomi = {シロタ, キンジ and サクラダ, ヨウスケ and ムラカミ, マサル and アカホリ, フミアキ and シロタ, マリコ}
}