{"created":"2023-06-19T07:19:09.396740+00:00","id":5066,"links":{},"metadata":{"_buckets":{"deposit":"efa474d2-63be-4ecd-a791-1913f831664b"},"_deposit":{"created_by":4,"id":"5066","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5066"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005066","sets":["17:253:315","31:181:494"]},"author_link":["21994","21995"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"281","bibliographicPageStart":"277","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"白色腐朽葉の菌種間,また同一の菌種内で互いに酵素化学的な性質の異なるアイソフォームが存在し,水酸化PCBの代謝性も異なることがわかった。T. versicolor産生ラッカーゼにおける水酸化PCB分解では,陰イオン交換樹脂未吸着両分との反応では,高塩赤化水酸化PCBは酸性側よりも中性側で分解率が高く,陰イオン交換樹脂吸着両分との反応では,低塩素化水酸化PCBについても同様の動向を示すことがわかった。さらに,同じ四塩素化水酸化PCBでも塩素の結合位置の違いにより,分解率に差が生じることが明らかとなった。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Several laccase isoforms showing the different enzymatic property were obtained from the culture fluid of some species of white-rot fungus, Trametes versicolor, T. cervina, T. conchifer and T. elegans. Each strain produced two kinds of laccases in the culture, one of which passed through the ion-exchange DEAE-Sepharose column at pH 5.0 (fraction A) and the other adsorbed the column (fraction B). Using 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate for laccase, the enzymatic property such as the optimum pH, the optimum temperature or the thermostability was investigated. As a result, every laccase from each strain had the higher activity in the acidic range, pH 3.8-4.4, and at the higher temperature, 60℃～80℃. Every laccase except the fraction B from T. conchifer and T. elegans showed the higher stability until 60℃; however, the fraction B still reserved the higher activity at 80℃. The degradation of hydroxyPCBs which are suspected of endocrine disruptors in the in vitro incubation with laccases was examined. Overall, the fraction A has a higher activity to reduce each level of hydroxyPCBs than the fraction B. In equally chlorinated 4-hydroxy-tetrachlorobiphenyl congeners, 4-hydroxy-2',3,5,5'-tetrachlorobiphenyl was more degraded than 4-hydroxy-2',3',4',5'-tetrachlorobiphenyl. It seems that the chlorine binding position is responsible for the rising susceptibility to the enzymatic degradation of hydroxyPCBs.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561554","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000120"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院環境保健学研究科環境保健科学専攻環境化学"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Environmental Chemistry, Course of Environmental Health Science, Graduate School of Environmental Health, Azabu university"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000120"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"其木, 茂則"},{"creatorName":"ソノキ, シゲノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sonoki, Shigenori","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-277.pdf","filesize":[{"value":"565.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-277.pdf","url":"https://az.repo.nii.ac.jp/record/5066/files/bull_jau_vol15-16-277.pdf"},"version_id":"5a189633-fb74-4c3c-b2a9-4158766f1be4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"環境汚染物質代謝に関与する新規ラッカーゼのスクリーニング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"環境汚染物質代謝に関与する新規ラッカーゼのスクリーニング"},{"subitem_title":"Screening of novel laccases degrading the environmental pollutants","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["315","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"5066","relation_version_is_last":true,"title":["環境汚染物質代謝に関与する新規ラッカーゼのスクリーニング"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:43:54.360380+00:00"}