@article{oai:az.repo.nii.ac.jp:00005066,
 author = {其木, 茂則 and Sonoki, Shigenori},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {白色腐朽葉の菌種間,また同一の菌種内で互いに酵素化学的な性質の異なるアイソフォームが存在し,水酸化PCBの代謝性も異なることがわかった。T. versicolor産生ラッカーゼにおける水酸化PCB分解では,陰イオン交換樹脂未吸着両分との反応では,高塩赤化水酸化PCBは酸性側よりも中性側で分解率が高く,陰イオン交換樹脂吸着両分との反応では,低塩素化水酸化PCBについても同様の動向を示すことがわかった。さらに,同じ四塩素化水酸化PCBでも塩素の結合位置の違いにより,分解率に差が生じることが明らかとなった。, Several laccase isoforms showing the different enzymatic property were obtained from the culture fluid of some species of white-rot fungus, Trametes versicolor, T. cervina, T. conchifer and T. elegans. Each strain produced two kinds of laccases in the culture, one of which passed through the ion-exchange DEAE-Sepharose column at pH 5.0 (fraction A) and the other adsorbed the column (fraction B). Using 2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the substrate for laccase, the enzymatic property such as the optimum pH, the optimum temperature or the thermostability was investigated. As a result, every laccase from each strain had the higher activity in the acidic range, pH 3.8-4.4, and at the higher temperature, 60℃～80℃. Every laccase except the fraction B from T. conchifer and T. elegans showed the higher stability until 60℃; however, the fraction B still reserved the higher activity at 80℃. The degradation of hydroxyPCBs which are suspected of endocrine disruptors in the in vitro incubation with laccases was examined. Overall, the fraction A has a higher activity to reduce each level of hydroxyPCBs than the fraction B. In equally chlorinated 4-hydroxy-tetrachlorobiphenyl congeners, 4-hydroxy-2',3,5,5'-tetrachlorobiphenyl was more degraded than 4-hydroxy-2',3',4',5'-tetrachlorobiphenyl. It seems that the chlorine binding position is responsible for the rising susceptibility to the enzymatic degradation of hydroxyPCBs., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {277--281},
 title = {環境汚染物質代謝に関与する新規ラッカーゼのスクリーニング},
 volume = {15/16},
 year = {2008},
 yomi = {ソノキ, シゲノリ}
}