@article{oai:az.repo.nii.ac.jp:00005060,
 author = {佐俣, 哲郎 and 其木, 茂則 and 秋山, 孝洋 and Samata, Tetsuro and Sonoki, Shigenori and Akiyama, Takahiro},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {1 軟体動物イワガキ殻体中に含まれる有機基質タンパク質の主要成分として,高度にリン酸化された52kDaの酸性糖タンパク質を固定した。2 この成分の機能解析を結晶形成実験により試みた。その結果,この成分が,単独では結晶形成を阻害するものの,不溶性有機基質の膜とともにインキュベートした場合に,平板状の方解石結晶が誘導されることが明らかになった。3 この結果から,リン酸化されたこの種の成分がCaC0_3の結晶多形の制御に関係していることが推定された。, Molluscan shell consists of complicated structure called shell microstructure, which is composed of two polymorphisms of calcium carbonate crystals, aragonite and calcite together with a tiny amount of the organic matrix (OM) secreted from the mantle tissue. Experiments have been conducted specifically to investigate the nacreous layer, which comprises aragonite, and has a specific geometry, in order to gain an understanding of the effect of OM on shell formation. In contrast to the nacreous layer, information about mineralization of the calcitic foliated layer is very limited. In spite of the recent report on the genes encoding the OM components in the foliated layer (Sarashina and Endo, 2001; Samata et al., in press), the process of the foliated layer formation is left a matter open. In the present study, we attempted to elucidate the process of the microstructure formation of the foliated layer, focusing on the function of the specific molecules in the oyster organic matrix by in vitro crystallization experiment. After decalcification by acetic acid, the soluble and insoluble OMs were extracted from the foliated layer of Crassostrea nippona. SDS-PAGE of these OM fractions resulted in a consecutive band with size of approximately 52 kDa, which could be visualized with stainsall, negative and methyl green stainings. The crystallization experiment was performed by adding OM components in the supersaturated crystallizing solution. When the 52 kDa component and the WISM were added to the crystallizing solution, calcite crystals with similar morphology of thin plate to those of the foliated layer of C.nippona were inducted on the WISM. These results may present new concepts that we think may well have implications to the biomineralization process of oyster shell., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {248--252},
 title = {海生無脊椎動物骨格における炭酸カルシウム結晶多形制御機構の解明},
 volume = {15/16},
 year = {2008},
 yomi = {サマタ, テツロウ and ソノキ, シゲノリ and アキヤマ, タカヒロ}
}