@article{oai:az.repo.nii.ac.jp:00005058,
 author = {福山, 正文 and 古畑, 勝則 and 大仲, 賢二 and Fukuyama, Masafumi and Furuhata, Katunori and Oonaka, Kenji},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {Campylobacter感染症に関する基礎的研究の一環として,2002～2006年にかけてヒト下痢便および鶏肉,鶏糞便から分解した株を用いて,血清型別,RAPD法による分子疫学的解析,薬剤感受性試験およびキノロン系薬剤に対する耐性株の遺伝子変異について検討を行ったところ,以下の成績が得られた。1)ヒト臨床由来53株と鶏由来102株の計155株を用いて血清型別を行ったところ,85株(54.8%)が16菌型に型別されたが,残りの70株(45.2%)は型別不能であった。型別された85株の内訳はY群に20株(12.9%)と最も多く,次にB群18株(11.6%),D群8株(5.2%)であった。由来別において,ヒト臨床由来ではB群に,鶏由来ではY群にそれぞれ多く型別された。2)RAPD法による分子疫学的検討を行ったところ,供試した155株中124株(80.0%)が類似度89%≦において1～30群に分類され,22群に22株(14.8%)と最も多く,次に18群10株価7%),16群8株(5.4%)などであった。由来別においては同一由来別に分類される傾向が強く認められ,由来の異なる株が共通した群へ分類される株は少数であった。3)ヒト下痢症由来53株の各種薬剤に対するMICの分布状況をMIC_<90>で比較したところ,GMが0.5μg/mlで最も感受性が高く,次にSMとLMが各2μg/ml,EMが4μg/ml,KMとCPが各8μg/ml,RXMが16μg/ml,MINOとCPFXが各32μg/ml,NAとNFLXが各128μg/ml,ABPC,PIPC,CEXおよびTCが各128<μg/mlの順であった。4)供試した15薬剤のうち10薬剤に対していずれかの株に耐性が認められた。その内訳はCEX99.4%, ABPC59.4%,NA40.6%,NFLX40.0%,TCとCPFX各39.4%,PIPC38.196,MINO30.3%,KM3.2%およびSM2.6%であった。5)薬剤耐性が認められた155株の耐性パターンの内訳は単剤耐性が28株(18.1%)と多剤耐性が127株(81.9%)であり,多剤耐性株が多いことを明らかにした。また,多剤耐性を示した耐性パターンではABPC/PIPC/CEXが最も多く,次にABPC/PIPC/CEX/TC/MINO/NA/NFLX/CPFXであった。6)キノロン系抗菌楽に対して耐性を示した44株のうち,43株(97.7%)がGyrAの変異(Thr-86→Ile)が認められたが,他の部位ではアミノ酸レベルでの変異は認められなかった。, As part of a basic study on campylobacteriosis, we performed serotyping, molecular-epidemiological analysis using the RAPD method, and drug sensitivity tests of strains isolated from human diarrheal feces, chicken meat, and chicken feces between 2002 and 2006, and investigated gene mutations in quinolone-resistant strains. 1) On serotyping of 53 human clinical isolates and 102 chicken meat-derived isolates, a total of 155 isolates, 16 types were identified in 85 isolates (54.8 %), but the remaining 70 isolates (45.2 %) could not be typed. Of the 85 typed isolates, 20 isolates (12.9 %) were typed Group Y, which was the most frequent, followed by 18 isolates typed Group B (11.6 %), and 8 isolates typed Group D (5.2 %). Regarding the origin, Groups B and Y were most frequently identified in human clinical and chicken meat-derived isolates, respectively. 2) On molecular-epidemiological analysis by RAPD, 124 of the 155 isolates (80.0 %) were classified into Groups 1-30 at a similarity of 89 % ≦, and 22 isolates were classified into Group 22 (14.8 %), which was the most frequent, followed by 10 isolates into Group 18 (6.7 %), and 8 isolates into Group 16 (5.4 %). Regarding the origin, isolates with the same origin tended to be classified into the same group, and only a few isolates were classified into groups consisting of isolates with a different origin. 3) MIC distribution in 53 human diarrhea-derived strains was compared among various drugs based on MIC_<90>. The MIC_<90> value of GM was 0.5 mg/ml, showing the highest sensitivity, followed by: SM and LM, 2 mg/ml; EM, 4 mg/ml; KM and CP, 8 mg/ml; RXM, 16 mg/ml; MINO and CPFX, 32 mg/ml; NA and NFLX, 128 mg/ml; and ABPC, PIPC, CEX, and TC, 128 mg/ml <. 4) There were isolates resistant to 10 of the 15 drugs tested: 99.4 % of the isolates were resistant to CEX, 59.4 % to ABPC, 40.6 % to NA, 40.0 % to NFLX, 39.4 % each to TC and CPFX, 38.1 % to PIPC, 30.3 % to MINO, 3.2 % to KM, and 2.6 % to SM. 5) Regarding the resistance patterns of the 155 isolates, 28 isolates showed single drug resistance (18.1 %), and 127 isolates showed multidrug resistance (81.9 %), revealing that many isolates were multidrug resistant. The most frequent multidrug resistance pattern was ABPC/PIPC/CEX, followed by ABPC/PIPC/CEXATC/MINO/NA/NFLX/CPFX. 6) GryA mutation (Thr86 → Ile) was detected in 43 (97.7 %) of 44 quinolone-resistant isolates, but no amino acid mutation was noted in the other regions., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {235--242},
 title = {ヒト下痢症由来および鶏肉由来Campylobacter属の疫学的研究},
 volume = {15/16},
 year = {2008},
 yomi = {フクヤマ, マサフミ and フルハタ, カツノリ and オオナカ, ケンジ}
}