| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2007-01-01 |
| タイトル |
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タイトル |
カンピロバクターのDNA分子識別 : Campylobacter lariの細胞膨張化致死毒素遺伝子オペロンのクローニングと構造解析 |
| タイトル |
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タイトル |
Cloning and structural analysis of full-length cytolethal distending toxin (CDT) gene operonfrom Campylobacter lari |
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言語 |
en |
| 言語 |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
| 記事種別 |
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内容記述タイプ |
Other |
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内容記述 |
特集 |
| Type |
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内容記述タイプ |
Other |
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内容記述 |
FEATURE ARTICLES |
| 著者 |
松田, 基夫
三田, 明弘
村山, 洋
Matsuda, Motoo
Sanda, Akihiro
Murayama, Ohoshi
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| 著者ID |
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000135 |
| 著者ID |
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000122 |
| 著者ID |
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000298 |
| Author ID |
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000135 |
| Author ID |
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000122 |
| Author ID |
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000298 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| Institution or Company |
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Graduate School of Environmental Health Sciences, Azabu University |
| Institution or Company |
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Graduate School of Environmental Health Sciences, Azabu University |
| Institution or Company |
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Graduate School of Environmental Health Sciences, Azabu University |
| Abstract |
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内容記述タイプ |
Other |
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内容記述 |
Approximately 2.5 k base pair (bp) amplicons encoding a cdt gene operon of about 2.3 kbp and two partial and putative open reading frames (ORFs) were identified with all the six urease-negative (UN) Campylobacter lari isolates obtained from different sources and in different countries using a novel PCR primer pair constructed in silico. Three closely spaced and possible ORFs for cdtA, B and C and two putative promoters and a hypothetically intrinsic p-independent transcription terminator were found in the operon. Each ORF commenced with an ATG start codon and terminated with a TGA stop codon for cdtA and B and a TAA for C. A possible overlap was identified between the four nucleotides including the stop codon (TGA) of the cdtA and the four nucleotides including the start codon (ATG) of the B, and the non-coding region of six bp occurred between cdtB and C. The start codons for the three cdt genes were preceded by Shine-Dalgarno sequences. Although nucleotide sequence differences were identified at seven loci in the cdtA gene, six B and two C, among seven isolates, including C. lari RM2100 reference strain, no polymorphic sites were demonstrated to occur in the putative promoters, hypothetically intrinsic transcription terminators and three ribosome binding sites among the seven isolates. Although no PCR amplicons were generated with more than 10 isolates of urease-positive thermophilic Campylobacter (UPTC) using the primer pair, C. lari consisting of UN C. lari and UPTC was suggested to carry a cdt gene operon in the genome. |
| 書誌情報 |
麻布大学雑誌
en : Journal of Azabu University
巻 15/16,
p. 229-234,
発行日 2008-03-31
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| 出版者 |
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出版者 |
麻布大学 |
| Publisher |
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出版者 |
Azabu University |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-5880 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11561545 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| text version |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| format |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
bull_jau_vol15-16-229.pdf (490.7 kB)
