@article{oai:az.repo.nii.ac.jp:00005054,
 author = {斑目, 広郎 and 西野, 佳以 and 井上, 真紀 and Madarame, Hiroo and Nishino, Yoshii and Inoue, Maki},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {1.ボルナ病ウイルス(BDV)のCRNP5株を脳内接種された仔ルイスラットを保育していた母ルイスラット1例が新生仔ラットへの脳内ウイルス接種から8週間後にボルナ病と考えられる重篤な神経症状が発症した。2.感染ラットの脳病変は非化膿性髄膜脳炎であった。中枢神経系では神経細胞を主体とするBDV陽性所見が認められた。3.BDV陽性細胞の分布の程度は嗅索を含む前頭葉,海馬(特にCA3領域)に強く,間脳,脳幹部,小脳,三叉神経節からも陽性所見が得られた。4.大脳皮質からは1.2×10^7FFU/gのウイルスが検出された。5.他のBDV感染仔ラットを保育した12匹の母ラット(ルイスラットおよびF344ラット)(8週間経過観察)については,神経症状ならびに脳病受け認められず,抗BDV抗体を用いた脳の免疫染色は全て陰性で,脳材料からもウイルスは回収されなかったが,抗BDV抗体測定結果では,3匹が陽性と判定された。6.12週間経過を観察した母ルイスラット7匹は発症しなかった。, Thirteen mother rats nursing BDV- inoculated pups were used. Within 24 hours after birth, the pups were inoculated with CRNP5 or CRP3 intracranially. Mother rats nursed their pups until the pups developed severe BD or weaned at postnatal day 28. Subsequently the mother rats were observed for about 8 weeks after giving a birth or showing clinical symptoms and developing severe BD. After observation, the mother rats were killed and histological studies and virus titration of the brain samples and antibody titration of the sera were performed. The expression of the antigens of BDV was also examined immunohistochemically. One mother rat developed neurological signs, suspected of indicating BD. This rat nursed pups infected with CRNP5 which died with neurological signs. Subsequently the mother rat developed neurological signs after the pups had died. The general condition of the mother deteriorated and the rat was killed and autopsied. Histopathologically, the rat had nonsuppurative meningoencephalitis. The lesions were distributed throughout the brain, but were more severe in the frontal part of the cerebral hemisphere, the rhinencephalon, and the hippocampus. In the hippocampus there were perivascular cuffings and neuronal degenerations with diffuse glyosis, especially in the hippocampal CA3 region. The regular cell layer arrangements had been lost in places. The neurons of the granular layers of the dentate gyrus had also degenerated and the neuronal cell arrangements were loosened. The positive reaction against BDV protein antibodies was distributed throughout the central nervous system, but was more intense in the rhinencephalon and the hippocampus. Cytologically, the positive reaction was observed mainly in the nucleus of the neurons. In the brain sample, the virus titer was 1.2x10^7 TCID_<50>/g. On the other hand, twelve mother rats without clinical symptoms had no histopathological changes and no positive reactions against BDV antibodies immunohistochemically in the brain, and BDV was not recovered from the brain. However, the anti-BDV antibody assay revealed 3 rats out of twelve had positive liters for BDV., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {214--218},
 title = {ボルナ病ウイルス感染ラットにおける母子伝播},
 volume = {15/16},
 year = {2008},
 yomi = {マダラメ, ヒロオ and ニシノ, ヨシイ and イノウエ, マキ}
}