{"created":"2023-06-19T07:19:08.794288+00:00","id":5052,"links":{},"metadata":{"_buckets":{"deposit":"6291a14a-23cc-4918-8070-dba89451e385"},"_deposit":{"created_by":4,"id":"5052","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5052"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005052","sets":["17:251:299","17:256:352","17:37:219","31:181:494"]},"author_link":["21917","21919","21914","21916","21915","21918"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"211","bibliographicPageStart":"208","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"乳腺組織由来のPTHrPは乳汁中へのCa輸送に関与する可能性があるため,乳腺組織におけるPTHrPのCa輸送に対する作用を,初代培養ウシ乳腺上皮細胞をコラーゲンコートしたメンブレン上で培養し,^<45>Caの輸送についてin vitroの系で検討した。非泌乳期のウシ乳腺組織をコラゲナーゼで消化し,プロラクチン,インスリン,ハイドロコルチゾンの存在下で培養して乳腺上皮細胞を得た。得られた乳腺上皮細胞をチャンバー法にて培養し,^<45>Caを内側あるいは外側のチャンバーに加え,PTHrPを添加した際の外側あるいは内側チャンバー溶液中の^<45>Ca放射活性を測定した。その結果,内側外側にかかわらず,PTHrPを添加した側の標識Caの放射活性は減少するのに対して,反対側の放射活性はそれに対応して必ずしも上昇するわけではなく,細胞内の標識Ca放射活性が上昇することが分かった。この結果は,PTHrPが乳腺上皮細胞のゴルジ装置へのCa輸送に作用することにより,血液中から乳汁中へのカルシウム輸送に関与していることを示唆するものであった。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Lactating mammary gland has been shown to produce large amounts of parathyroid hormone-related protein (PTHrP), and high levels of PTHrP have been measured in milk; however, the involvement of PTHrP to Ca transport into milk is unknown. Here we report that PTHrP is very likely to participate with active Ca transport to mammary epithelial cells.Bovine mammary epithelial cells were cultured on collagen coated membrane (Transwell, Corning, USA). MBP-bovine PTHrP[1-141](10, 100, and 1,000 fM), CaCl_2 (2.5 mM) and ^<45>Ca (0.05 μCi/100 μl) were added inside or outside of the membrane and cells were incubated for 1 hour (37℃, 5 % CC_2). Both sides of solution and cells were collected and measured ^<45>Ca radio activity. Regardless of the side of PTHrP addition, radio activities of ^<45>Ca were decreased in the added side and not necessarily increased in the opposite side, they were increased in cultured cells. These results suggest that PTHrP produced in the bovine mammary gland participate Ca transport from circulation to milk via Golgi apparatus in the mammary alveolar epithelial cells.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561540","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000070"},{"subitem_text_value":"000329"},{"subitem_text_value":"000277"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院"},{"subitem_text_value":"麻布大学大学院"},{"subitem_text_value":"麻布大学"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"AZABU University, Graduate School of Veterinary Science"},{"subitem_text_value":"AZABU University, Graduate School of Veterinary Science"},{"subitem_text_value":"AZABU University, School of Veterinary Medicine"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000070"},{"subitem_text_value":"000329"},{"subitem_text_value":"000277"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"和田, 恭則"},{"creatorName":"ワダ, ヤスノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伊東, 正吾"},{"creatorName":"イトウ, セイゴ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"恩田, 賢"},{"creatorName":"オンダ, ケン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Yasunori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ito, Seigo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Onda, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-208.pdf","filesize":[{"value":"586.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-208.pdf","url":"https://az.repo.nii.ac.jp/record/5052/files/bull_jau_vol15-16-208.pdf"},"version_id":"fb7bb4b8-ea16-4719-80de-0e85fe54fac1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ウシ乳腺組織由来の牛乳中副甲状腺ホルモン関連タンパク質(Parathyroid hormone-related protein; PTHrP)とPTHrP由来ペプチドの作用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウシ乳腺組織由来の牛乳中副甲状腺ホルモン関連タンパク質(Parathyroid hormone-related protein; PTHrP)とPTHrP由来ペプチドの作用"},{"subitem_title":"Function of bovine milk Parathyroid hormone-related protein (PTHrP)","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["219","299","352","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"5052","relation_version_is_last":true,"title":["ウシ乳腺組織由来の牛乳中副甲状腺ホルモン関連タンパク質(Parathyroid hormone-related protein; PTHrP)とPTHrP由来ペプチドの作用"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:43:34.486405+00:00"}