@article{oai:az.repo.nii.ac.jp:00005040,
 author = {岡谷, 友三アレシヤンドレ and 村上, 賢 and 森田, 英利 and 加藤, 行男 and Okatani, Alexandre T. and Murakami, Masaru and Morita, Hidetoshi and Kato, Yukio},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {本研究では,LAMP法を用い,gyrB遺伝子を標的とした豚丹毒菌の迅速な検出法の開発を行った。供試菌株としてE. rhusiopathiae, E. tonsillarum, Erysipelothrix spp血清型13型および血清型18型を用いた。各菌株のDNAを抽出,複数のプライマーを用いてPCRで標的遺伝子の増幅を試みた。供試したPCRプライマーのうち,プライマーUP1/UP2rでE. rhusiopathiae, E. tonsillarumおよび血清型13型で増幅産物が得られ,血清型18型の菌株からは前述のプライマーを基に設定したプライマーで増幅産物が得られた。PCRで得られた増幅産物を精製,遺伝子配列の決定を行い,LAMPプライマーを設定した。設定されたLAMPプライマーのうち,1つのプライマーセットでは供試した4菌株すべてが検出可能で,また,他のプライマーセットではE. tonsillarumおよび血清型13型の菌株の検出が可能であった。, The aim of this study was to develop a rapid detection method of Erysipelothrix species. DNA of E. rhusiopathiae, E. tonsillarum, and Erysipelothrix spp. serovar 13 and serovar 18 were extracted and submitted for detection and sequencing of the gyrB gene. Among the primers tested for amplifying the gyrB, the universal primer set UP1UP2r showed the best result, and a DNA fragment were obtained from the E. rhusiopathiae, E. tonsillarum and Erysipelothrix spp. serovar 13. DNA amplification band from Erysipelothrix spp. serovar 18 was obtained by a primer derived from the above primer set. The obtained fragments were introduced in the pGEM-T Easy Vector and submitted for sequencing. LAMP primers sets were generated from the obtained sequence, and, among the generated primer sets, one primer set was able to detect the 4 strains tested, and another set was able to detect the E. tonsillarum and the strain of serovar 13., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {164--167},
 title = {豚丹毒菌の迅速診断法の開発},
 volume = {15/16},
 year = {2008},
 yomi = {オカタニ, トモミツアレシヤンドレ and ムラカミ, マサル and モリタ, ヒデトシ and カトウ, ユキオ}
}