{"created":"2023-06-19T07:19:08.106334+00:00","id":5036,"links":{},"metadata":{"_buckets":{"deposit":"9ea42ea0-7f57-4030-8a13-59ea2aeb2de5"},"_deposit":{"created_by":4,"id":"5036","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5036"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005036","sets":["17:37:227","17:40:280","17:40:281","31:181:494"]},"author_link":["21807","21811","21808","21812","21810","21809"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"148","bibliographicPageStart":"145","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マスト細胞は細菌感染,特にグラム陰性菌による感染に対し重要な役割を果たしていることは良く知られている。マスト細胞はLPSがトール様受容体に結合することによって活性化するが,そのマスト細胞の機能的な変化はまだ充分に説明されていない。本研究では,TLRを介してLPS刺激を受けたマスト細胞におけ種々なサイトカインとその役割を調査した。LPS刺激によってマスト細胞では,前炎症性サイトカインおよびTh2サイトカインであるTNFα, IL-1β, MIP-1, MCP-1, IL-4, Il-6, IL-13に加え,マスト細胞の増殖因子であるIL-9のmRNAの発現が観察された。IL-9 mRNA発現は,LPS濃度依存性に発現が増加し,その受容体であるIL-9レセプターは持続的に強い発現がマスト細胞で認められた。次にマスト細胞増殖に対するIL-9の効果をMTT法および細胞測定法で調査した。IL-9単独ではマスト細胞の増殖は見られなかったが,IL-3, SCFおよびその両者との混合培養では相乗的なマスト細胞の増殖が見られた。これらの結果から,LPSにより誘導されるマスト細胞のIL-9は感染局所におけるマスト細胞の増殖に関連することが強く示唆された。それらの機構は,自然免疫あるいは適応免疫において重要な役割をはたしているかもしれない。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"It is well known that mast cell play critical role in bacterial infection especially due to gram negative bacteria. Mast cell was activated by binding LPS to Toll-like receptor, however the mast cell functional changes have not been still unexplained. In present study, we investigated the cytokine profiles and its roles in mast cell stimulated with LPS through TLR signaling. We observed that in addition to the proinflamatory and Th2 cytokines, TNF-α, IL-1β, MIP-1, MCP-1, IL-4, IL-6 and L-13, LPS induced the mRNA expression of IL-9 which is recognized mast cell proliferation capacity. The IL-9 mRNA expression indicated LPS-dose dependent matter and IL-9R mRNA shown constitutively high level. Next we investigated the effect of IL-9 for the proliferation of mast cell by MTT and cell count assay. In both of assay, IL-9 was not shown mast cell proliferation. In contrast, IL-9 with IL-3 or SCF and both induced synergistically increasing mast cell. Taken together, these observations provide strong evidence indicating IL-9 response of mast cell to LPS involves mast cell proliferation in infectious site. These mechanisms may play critical role in mast cell of both innate and adaptive immunity.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561524","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000065"},{"subitem_text_value":"000259"},{"subitem_text_value":"000247"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医免疫学研究室"},{"subitem_text_value":"麻布大学分子生物学研究室"},{"subitem_text_value":"麻布大学食品科学研究室"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Laboratoty of Veterinary Immunology, School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Laboratoty of Moleculorbiology, School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Laboratoty of Food Science, School of Veterinary Medicine, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000065"},{"subitem_text_value":"000259"},{"subitem_text_value":"000247"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"池田, 輝雄"},{"creatorName":"イケダ, テルオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 賢"},{"creatorName":"ムラカミ, マサル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森田, 英利"},{"creatorName":"モリタ, ヒデトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikeda, Teruo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murakami, Masaru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morita, Hidetoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-145.pdf","filesize":[{"value":"353.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-145.pdf","url":"https://az.repo.nii.ac.jp/record/5036/files/bull_jau_vol15-16-145.pdf"},"version_id":"a2599a4f-a3bd-47c8-a909-cbb54b745876"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"免疫応答におけるマスト細胞活性化機構に関する研究 : Toll-like receptorによるマスト細胞の活性化について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"免疫応答におけるマスト細胞活性化機構に関する研究 : Toll-like receptorによるマスト細胞の活性化について"},{"subitem_title":"Activating mechamism of mast cell in immuno-responses : mast cell activation due to Toll-like receptor","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["227","280","281","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"5036","relation_version_is_last":true,"title":["免疫応答におけるマスト細胞活性化機構に関する研究 : Toll-like receptorによるマスト細胞の活性化について"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:44:16.558921+00:00"}