{"created":"2023-06-19T07:19:07.974956+00:00","id":5033,"links":{},"metadata":{"_buckets":{"deposit":"bb819a3c-4014-4508-a0d6-6bbba8c43a9b"},"_deposit":{"created_by":4,"id":"5033","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5033"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005033","sets":["17:37:226","17:38:265","31:181:494"]},"author_link":["21795","21800","21790","21783","21784","21792","21791","21799","21789","21785","21796","21788","21798","21793","21786","21794","21787","21797"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"137","bibliographicPageStart":"134","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ビオチン欠乏時および回復後のラット海馬歯状回のニューロジェネシスについて免疫組織化学的に検証した。Wistar系雄ラットを用いた。離乳直後からビオチン欠乏飼料を与え,ペアフィーディング(以下PF)を行い,ビオチン欠乏群(BD群)には生理食塩水を,対照群(BS群)にはビオチン(200μg/kg)をそれぞれ毎日腹腔内投与した。半数(BDI群・BS I群)は5週間後に剖検し,残りの半数(BD II群・BS II群)はBD群ヘビオチンを投与し4週間回復させた後に剖検し,脳を摘出した。脳は海馬の同一部位を厚さ20μmの凍結切片を個体ごとに6枚作製し,神経細胞検マーカーである抗Neuronal Nuclei (NeuN)抗体と抗BrdU抗体を用いた二重免疫染色を施し,BrdU陽性細胞数およびBrdU/NeuN陽性細胞数を計測した。BD I群・BS I群において,BrdU陽性細胞数とBrdU/NeuN陽性細胞数のどちらも有意に減少していた(P<0.001)。さらに,回後期を設けたBD II群・BS II群においては,BrdU陽性細胞数とBrdU/NeuN陽性細胞数は,ほぼ同様の値を示した。ビオチン欠乏状態では海馬歯状回のニューロジェネシスは抑制され,逆にビオチン欠乏状態回復後では加齢に伴う新生細胞数の減少は観察されたものの,対照群のそれと比べて同等のレベルまで改善することが確認された。このことから,ビオチンは神経細胞の新生および分化,つまり脳機能の維持・発達に重要な役割を果たしているものと推察された。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"We previously confirmed that learning ability of a biotin-deficient (BD) rat decreased and LTP of its hippocampus was suppressed. In this study, the neurogenesis of hippocampal dentate gyruses of rats in BD conditions were investigated immunohistochemically. Wistar strain rats, 3-week-old, were fed BD diet. BD group was subjected to daily i.p. injection of saline for 5 weeks. Control (BS) group received same diet with daily injection of biotin (200 μg/kg, i.p.). The rats of the BD and BS groups were administered 5-Bromo-2'-deoxyuridine (BrdU, 50 mg/kg, i.p.) every 5 days, at a total of 4 times before autopsy. After exsanguination under anesthesia, the brains were removed, fixed with 4 % para-formaldehyde, sectioned 20 μm thick, and stained immunohistochemically with anti-neuronal nuclei (NeuN) and anti-BrdU antibodies. The numbers of BrdU positive cells of BD and BS were 39.1 ± 1.0 and 53.0 ± 0.8, respectively, and the numbers of BrdU/NeuN positive cells were 26.8 ± 0.7 and 39.3 ± 0.6, respectively. Both numbers of BrdU and BrdU/NeuN positive cells of the BD group showed significantly small values (p < 0.001). The neurogenesis of the hippocampus was suppressed in the BD condition. This suggests that biotin has an important role in the development and maintenance of brain functions.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561521","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000274"},{"subitem_text_value":"-"},{"subitem_text_value":"000063"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医学部実験動物学研究室"},{"subitem_text_value":"麻布大学獣医学部実験動物学研究室"},{"subitem_text_value":"日本動物愛玩協会"},{"subitem_text_value":"南大谷クリニック"},{"subitem_text_value":"三菱化学生命科学研究所"},{"subitem_text_value":"本荘第一病院"},{"subitem_text_value":"麻布大学獣医学部動物繁殖学研究室"},{"subitem_text_value":"麻布大学獣医学部実験動物学研究室"},{"subitem_text_value":"麻布大学獣医学部実験動物学研究室"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Lab. of Anim. Sci., Azabu Univ."},{"subitem_text_value":"Lab. of Anim. Sci., Azabu Univ."},{"subitem_text_value":"Pet Care Assoc."},{"subitem_text_value":"Minamiohya Clin."},{"subitem_text_value":"Mitubishikagaku Inst."},{"subitem_text_value":"Honjo Daiichi Hosp."},{"subitem_text_value":"Lab. of Anim. Reprod., Azabu Univ."},{"subitem_text_value":"Lab. of Anim. Sci., Azabu Univ."},{"subitem_text_value":"Lab. of Anim. Sci., Azabu Univ."}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000274"},{"subitem_text_value":"-"},{"subitem_text_value":"000063"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"遠藤, 伸"},{"creatorName":"エンドウ, シン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"吉田, しおり"},{"creatorName":"ヨシダ, シオリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"崎田, 克康"},{"creatorName":"サキタ, カツヤス","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"永井, 哲志"},{"creatorName":"ナガイ, テツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"上條, 信一"},{"creatorName":"カミジョウ, シンイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"前橋, 賢"},{"creatorName":"マエバシ, マサル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"柏崎, 直巳"},{"creatorName":"カシワザキ, ナオミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"二宮, 博義"},{"creatorName":"ニノミヤ, ヒロヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"猪股, 智夫"},{"creatorName":"イノマタ, トモオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Endo, Shin","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Shiori","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakita, Katsuyasu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagai, Tetsushi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamijo, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maebashi, Masaru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kashiwazaki, Naomi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ninomiya, Hiroyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Inomata, Tomo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-134.pdf","filesize":[{"value":"293.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-134.pdf","url":"https://az.repo.nii.ac.jp/record/5033/files/bull_jau_vol15-16-134.pdf"},"version_id":"7ee579ef-8238-447b-b153-1edfcb0c4028"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ビオチン欠乏がラット海馬に及ぼす影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ビオチン欠乏がラット海馬に及ぼす影響"},{"subitem_title":"Effects of biotin deficiency on rat hippocampuses","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["226","265","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"5033","relation_version_is_last":true,"title":["ビオチン欠乏がラット海馬に及ぼす影響"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:44:32.431693+00:00"}