{"created":"2023-06-19T07:19:07.799453+00:00","id":5029,"links":{},"metadata":{"_buckets":{"deposit":"febadadc-ef98-4798-859c-c17726bc136e"},"_deposit":{"created_by":4,"id":"5029","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5029"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00005029","sets":["17:37:227","17:40:280","31:181:494"]},"author_link":["21763","21766","21764","21767","21765","21768"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"109","bibliographicPageStart":"107","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"組織特異的転写因子であるMitfには,exon 1のalternative splicingによりN末領域のみの配列が異なる少なくとも9種類のアイソフォーム(Mitf-A, -B, -C, -D, -E, -H, -J, -M, -mc)がある。Mitf-A, -H, -Mではさらに,exon 6a(6個のアミノ酸をコードする)のalternative splicingによるvariant (6a+bと6b)が存在することが知られている。本研究では,色素細胞,マスト細胞,マクロファージ系細胞と心筋細胞でのMitfの各アイソフォームとvariantの遺伝子発現を調べ,細胞種によって異なるアイソフォームの発現が見られることを示した。また,これらの細胞に存在するMitf -E, -J, -mcのアイソフォームにも6a+bと6bのvariantがあることを見つけた。PCR-RFLP解析により,これら6a+bと6bのvariantの発現量はアイソフォームや細胞種に関係なく,ほぼ同じであることがわかった。次に,これらのvariantの転写活性機能を比較するため,ルシフェラーゼレポーターアッセイを行った。Mitfの応答遺伝子であるTyrosinase, Tyrp-1, Mmcp-6, PAI-1のそれぞれの制御領域配列をもつルシフェラーゼレポーターを構築し,Mitf-A, -D/E, -Mそれぞれの6a+bと6bのvariantを強制発現させた。Tyrosinase制御領域配列をもつレポーターに対して6a+bは6aのvariantに比べてより強い促進活性を示したが,Tyrp-1, Mmcp-6, PAI-1のレポーターに対しては,Tyrp-1レポーターへのMitf -D/Eのvariantの活性を除いて,6a+bと6bのvariantで活性に違いは見られなかった。以上,細胞に依存したMitfアイソフォームの多様な遺伝子発現パターンと異なる転写活性を示した。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Microphthalmia-associated transcription factor (Mitf) is a tissue-specific transcription factor. At least nine distinct mouse isoform mRNAs are encoded by alternative splicing of the first exon of Mitf (Mitf-A, -B, -C, -D, -E, -H, -J, -M and -mc), while exons 2 to 9 of all Mitf isoforms examined to date are identical. In addition, alternative splice variants of exon 6a encoding 6 amino acid proximal to the basic region of the protein are known in Mitf-A, -H, and -M. In this study, we identified alternative splice variants of exon 6a in other Mift isoforms (Mitf-E, -J and -mc) in melanocytes, mast cells, macrophages, and heart. We also compared the transcriptional activity of Mitf variants containing exon 6a to that of Mitf variants that did not contain exon 6a. PCR-RFLP analysis revealed that expression of Mitf with exon 6a was comparable with that of Mitf without exon 6a, irrespective of the specificity of the first exon, or cell type, although Mitf isoforms with different first exons were expressed in a cell type-dependent manner. Luciferase-based reporter assays revealed that transcription of Tyrosinase, which is known Mitf-regulated gene, was elicited more efficiently by expression of Mitf isoforms containing exon 6a, compared to isoforms that did not contain exon 6a. However, when transcription of Tyrp-1, Mmcp-6 and Pai-1 was examined, significant differences were detected between Mitf isoforms with exon 6a and those without exon 6a, except for Tyrp-1 transcription by Mitf-D/E isoform. These results reveal a diverse pattern of gene expression and different transcriptional activities of Mitf isoforms, suggesting discrete regulation of gene transcription in specific tissues by Mitf.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561517","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000259"},{"subitem_text_value":"-"},{"subitem_text_value":"000065"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学獣医学部"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Azabu University School of Veterinary Medicine"},{"subitem_text_value":"Azabu University School of Veterinary Medicine"},{"subitem_text_value":"Azabu University School of Veterinary Medicine"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000259"},{"subitem_text_value":"-"},{"subitem_text_value":"000065"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"村上, 賢"},{"creatorName":"ムラカミ, マサル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"21763","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"舟場, 正幸"},{"creatorName":"フナバ, マサユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"21764","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"池田, 輝雄"},{"creatorName":"イケダ, テルオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"21765","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murakami, Masaru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21766","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Funaba, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21767","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ikeda, Teruo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"21768","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-107.pdf","filesize":[{"value":"234.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-107.pdf","url":"https://az.repo.nii.ac.jp/record/5029/files/bull_jau_vol15-16-107.pdf"},"version_id":"3a13c870-ccff-4dfb-b1af-5cb8c031ef6c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"多様な分化制御因子である転写因子Mitfの役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"多様な分化制御因子である転写因子Mitfの役割"},{"subitem_title":"The role of microphthalmia-associated transcription factor (Mitf), a tissue-specific transcription factor, which acts as a master regulator of diverse cell differentiation","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["227","280","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"5029","relation_version_is_last":true,"title":["多様な分化制御因子である転写因子Mitfの役割"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:44:19.071322+00:00"}