{"created":"2023-06-19T07:19:05.795795+00:00","id":4983,"links":{},"metadata":{"_buckets":{"deposit":"c2148209-cac7-4351-ba2b-4285a69234be"},"_deposit":{"created_by":4,"id":"4983","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4983"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004983","sets":["17:40:280","31:181:494"]},"author_link":["21629","21631","21632","21630","21627","21628"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"15/16","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"The Japanese silver crucian carp (so-called ginbuna, Carassius auratus langsdrofi) has two reproduction systems; one is a sexual reproduction practiced by diploid individuals and the other is gynogenetic reproduction by triploid individuals. In this study, AFLP (Amplified Fragment Length Polymorphism) analysis was carried out to isolate and characterize genomic DNA markers for triploid ginbuna, as a step toward revealing the genomic makeup and origin of triploid ginbuna. Two valuable DNA markers were isolated from 14 fragments cloned from 3 AFLP fragments in the triploid ginbuna genome. Specific PCR analyses to these DNA markers and their sequencing analyses allowed us to divide the triploid ginbuna DNA into 6 genomic types (type A～F). The PCR products from many triploid ginbuna individuals comprising type A, C, D and E were shared with those from diploid ginbuna and those from the continental silver crucian carp, C. a. gibelio. The triploid ginbuna of type B appears to be derived from the gibelio crucian carp. These data provide additional evidence for the multiple origins of the triploid ginbuna and the genomic contribution made by the diploid ginbuna and the gibelio crucian carp to the triploid hybrid ginbuna.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"原著","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"ORIGINAL ARTICLE","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561471","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000259"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医学部分子生物学研究室"},{"subitem_text_value":"麻布大学獣医学部分子生物学研究室"},{"subitem_text_value":"麻布大学獣医学部分子生物学研究室"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Laboratory of Molecular Biology, School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Laboratory of Molecular Biology, School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Laboratory of Molecular Biology, School of Veterinary Medicine, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000259"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高瀬, 有加里"},{"creatorName":"タカセ, ユカリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"藤谷, 英男"},{"creatorName":"フジタニ, ヒデオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 賢"},{"creatorName":"ムラカミ, マサル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKASE, Yukari","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJITANI, Hideo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MURAKAMI, Masaru","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-01-01"}],"displaytype":"detail","filename":"bull_jau_vol15-16-001.pdf","filesize":[{"value":"822.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol15-16-001.pdf","url":"https://az.repo.nii.ac.jp/record/4983/files/bull_jau_vol15-16-001.pdf"},"version_id":"bb684f64-d1d4-430f-b8a9-afddcc0dd06e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AFLP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"triploid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ginbuna","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"crucian carp","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gynogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"AFLP (Amplified Fragment Length Polymorphism:増幅断片長多型)解析による3倍性ギンブナ(Carassius auratus langsdorfi)に特徴的なゲノムマーカーの探索","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"AFLP (Amplified Fragment Length Polymorphism:増幅断片長多型)解析による3倍性ギンブナ(Carassius auratus langsdorfi)に特徴的なゲノムマーカーの探索"},{"subitem_title":"Isolation and Characterization of Genomic DNA Markers from the Gynogenetic Triploid Ginbuna (Carassius auratus langsdrofi) by Amplified Fragment Length Polymorphism Analysis","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["280","494"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-01-01"},"publish_date":"2007-01-01","publish_status":"0","recid":"4983","relation_version_is_last":true,"title":["AFLP (Amplified Fragment Length Polymorphism:増幅断片長多型)解析による3倍性ギンブナ(Carassius auratus langsdorfi)に特徴的なゲノムマーカーの探索"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:44:20.456532+00:00"}