@article{oai:az.repo.nii.ac.jp:00004858,
 author = {斑目, 広郎 and 西野, 佳以 and 井上, 真紀 and Madarame, Hiroo and Nishino, Yoshii and Inoue, Maki},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {1.F344ラットの新生仔脳内感染でも,BDV CRNP5株(マウス順化株)とCRP3株(ラット順化株)の間で神経症状と中枢神経系病変に差異が認められた。CRNP5株接種ラットは重度の神経症状を示し,CRP3株接種仔ラットと比較してより重度の組織病変が脳全体に認められたが,特に海馬領域に病変を認めた。一方,CRP3株接種群には全実験期間(20日間)を通じて目視による症状は出現せず,脳の組織病変も殆ど認められなかった。2.BDV感染ラットにおける神経症状,運動機能障害と行動異常を数値化するための試験と実験条件を選定した。, A pilot study for investigating human neurological and psychiatric diseases based on neonatal rat infection with Borna disease virus (BDV) was described. The histopathological changes of the central nervous system and clinical disease of rats infected with two related BDV variants, CRP3 (rat-adapted variant) or CRNP5 (mouse-adapted variant) in Fischer 344 rats were investigated for 20 days. Infected rat pups were scored for incidence and severity of BD (the severity of disease in rats ranked on a scale of 0 to 3+) and examined histopathologically. In addition, we selected the protocol design and testing procedures for assessing behavior of BDV infected rat neonates. Compared to newborn rats inoculated with CRP3, newborn rats inoculated with CRNP5 had more severe histopathological changes in the central nervous system, mainly in the hippocampus. There was no evidence of encephalitis in either CRP3 or CNRP5 infection. In CRNP5 infection, there were severe degeneration of the pyramidal neurons of the hippocampus and extensive hippocampal gliosis. On the contrary, CRP3-inoculated newborn rats had little histopathological changes in the hippocampus by day 20 p. i.. As for the severity of BD, all neonatal rats inoculated with CRNP5 were scored 1+ by day 12 p. i.. On the day 16 p. i., six out of eight rats were scored 2+. By day 20 p. i., all rats were ranked 2+ or 3+, and one infected rat died. On the other hand, CRP3-inoculated newborn rats showed no apparent neurological signs by day 20 p. i.. In addition, we chose a) body lighting, b) negative geotropism, c) grasping response, d) fore limb and hind limb placing and e) bar holding test for the behavioral tests by postnatal days (PND) 20. From rats at PND25-28 onwards, beam walking (bar crossing) tests and open field tests were selected for assessment of the abnormal behavior., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {196--198},
 title = {ボルナ病ウイルス感染動物を用いたヒト内因性精神疾患モデル動物の作出},
 volume = {11/12},
 year = {2006},
 yomi = {マダラメ, ヒロオ and ニシノ, ヨシイ and イノウエ, マキ}
}