@article{oai:az.repo.nii.ac.jp:00004846,
 author = {遠藤, 明仁 and 岩本, 浩昭 and 白鳥, 千春 and オカタニ, A.トモミツ and 村上, 賢 and 加藤, 行男 and 長山, 昌広 and 岡田, 早苗 and 森田, 英利 and Endo, Akihito and Iwamoto, Hiroaki and Shiratori, Chiharu and Okatani, Tomomitsu A. and Murakami, Masaru and Kato, Yukio and Nagayama, Masahiro and Okada, Sanae and Morita, Hidetoshi},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {本研究では,培養法とPCR-変性剤農度勾配ゲル電気泳動(DGGE)法を用いて,健常なサラブレッドの腸内フローラを解析した。2頭の競走馬について,17種類の選択培地を用いて培養法による腸内フローラ解析を行った。それら2頭のウマを含む6頭については,細菌に対するユニヴァーサルプライマーと,Lactobacillus属,Enterococcus属およびBifidobacterium属特異的プライマーを用いたPCR-DGGE法での腸内フローラ解析を行った。PCR-DGGE解析により,すべてのウマからLactobacillus equi,Lactobacillus近縁種,Lactobacillus gastricus近縁種,Stretococcus bovis/Strepotococcus equinusが検出された。single PCRでは,PCRバイアスの関係からBifidobacterium属を検出できなかった。そこで,すべてのウマに対しnested PCRを試みたところ,3頭のウマでBifidobacterium boum,Bifidobacterium urinalis近縁種とParascardovia denticolensを検出した。B. boumは,培養法で生菌として分離した。一方,3頭のウマからLactobacillus属として L. equi, L. salivarus, Lactobacillus crispatus, Lactobacillus ruminis, Lactobacillus mucosae, Lactobacillus reuteri, Lactobacillus jonsonii, Lactobacillus thermotolerans, Lactobacillus delbrueckii と Lactobacillus vitulinus近縁種を分離した。これらの結果から,現役競走馬の特異的フローラとして,Bifidobacterium属の構成比が他の哺乳動物に比べて低いことが示された。, We analyzed the gastrointestinal flora of active racehorses by culture method and determined by PCR-denaturing gradient gel electrophoresis (DGGE). One active racehorse feces was analyzed by using 17 selective media as the culture method. The bacterial flora, and Lactobacillus, Enterococcus and Bifidobacterium flora in the feces of 6 healthy, actively racing Thoroughbreds were used by PCR-denaturing gradient gel electrophoresis (DGGE) with primer sets universal and specific for each group, respectively. PCR-DGGE analyses of the feces showed that Lactobacillus equi, an organism phylogenetically related to Lactobacillus salivarius, and a strain phylogenetically related to Lactobacillus gastricus were present in almost all of the fecal samples tested, and Streptococcus bovis/Streptococcus equinus was the predominant representative of the Enterococcus group. Single PCR of the samples failed to detect any members of the Bifidobacterium group, but nested PCR identified Bifidobacterium boum, two isolates phylogenetically related to Bifidobacterium urinalis, and Parascardovia denticolens in 3 of the 6 horses tested. B. boum was isolated by the culture method, although the population of Bifidobacterium was very low. On the other hand, L. equi, L. salivarius, Lactobacillus crispatus, Lactobacillus ruminis, Lactobacillus mucosae, Lactobacillus reuteri, Lactobacillus jonsonii, Lactobacillus thermotolerans, Lactobacillus delbrueckii and Lactobacillus vitulinus were isolated as Lactobacillus group from the feces of 3 healthy, actively racing Thoroughbreds. These results indicate that the lactic acid bacterial and bifidobacterial distributions in horse feces are unique because of the presence of species specific to horses and because of the dearth of Bifidobacterium isolates., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {140--145},
 title = {培養法とPCR-DGGE法による現役競走馬(サラブレッド)の腸内フローラ解析},
 volume = {11/12},
 year = {2006},
 yomi = {エンドウ, アキヒト and イワモト, ヒロアキ and シラトリ, チハル and /, ムラカミ マサル and カトウ, ユキオ and ナガヤマ, マサヒロ and オカダ, サナエ and モリタ, ヒデトシ}
}