{"created":"2023-06-19T07:19:03.838616+00:00","id":4839,"links":{},"metadata":{"_buckets":{"deposit":"2a81946b-0893-4fb2-977c-dbe715215b3c"},"_deposit":{"created_by":4,"id":"4839","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4839"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004839","sets":["31:181:508"]},"author_link":["21045","21047","21046","21044","21048","21049"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"109","bibliographicPageStart":"106","bibliographicVolumeNumber":"11/12","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"以前の研究において,TGF-βファミリーに属するactivin A と TGF-β_1は,ともに,骨髄由来の培養マスト細胞(BMMC)の機能-細胞の成熟(マスト細胞プロテアーゼ(mmcps)の発現増),細胞増殖抑制や遊走-を同じように変化させることを明らかにしている。現時点において,受容体結合以降の細胞内情報伝達機構に関して,activin と TGF-βにおいて差はない。本研究では,アゴニストとしてTGF-β_1を用いて,TGF-β_1によるBMMCの機能変化におけるSmad3 と P38MAPキナーゼの関与を調べた。TGF-β_1処理したBMMCではP38キナーゼは2時間以内にリン酸化され,リン酸化は少なくとも24時間継続した。TGF-β_1によるBMMCの機能変化におけるp38キナーゼの関与の仕方は,機能に依存した。すなわち,TGF-β_1によるmmcp-1の誘導や遊走に対してP38キナーゼは必要なのに対して,mmcp-7の誘導や細胞増殖の抑制に対しては必要ではなかった。野生型BMMCと比較して,Smad3欠損BMMCではTGF-β_1による細胞増殖抑制程度は小さくなった。野生型BMMCでは40fMのTGF-β_1に遊走する活性があったが,Smad3欠損BMMCではTGF-β_1に対する遊走能は消失した。以上の結果,BMMCにおいてTGF-β_1が引き起こす細胞の反応においてp38キナーゼとSmad3の関与の仕方は機能によって変化することが明らかになった。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Previous studies have indicated that activin A and TGF-β_1, members of the TGF-β family, modulate functions of bone marrow-derived cultured mast cells (BMMC) ; cell maturation (up-regulation of mouse mast cell proteases (mmcps)), growth arrest and migration. At present activin signaling is indistinguishable from TGF-β signaling at the signaling molecule level of downstream of receptor binding. In the present study, the roles of p38 MAP kinase and Smad3 in TGF-β-mediated cell responses in BMMC were explored. Treating BMMC with TGF-β_1-induced the phosphorylation of p38 within 2 h and persisted for 24 h. The involvement of p38 in TGF-β_1-induced cell responses depended upon mast cell functions ; it was necessary for up-regulation of mmcp-1 and migration, but not for up-regulation of mmcp-7 and inhibition of MTT uptake and reduction. The decrease in MTT uptake and reduction in response to TGF-β_1 treatment was smaller in Smad3-deficient BMMC compared to wild-type BMMC. Maximal migration was detected at a TGF-β_1 concentration of 40 fM in wild-type BMMC, whereas TGF-β_1-induced migration was absent in Smad3-deficient BMMC. Thus, the roles of p38 and Smad3 are different among TGF-β-mediated cell responses in BMMC.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学獣医学部栄養学研究室"},{"subitem_text_value":"麻布大学獣医学部微生物学第一研究室"},{"subitem_text_value":"麻布大学獣医学部分子生物学研究室"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Laboratories of Nutrition, Azabu University School of Veterinary Medicine"},{"subitem_text_value":"Veterinary Microbiology I, Azabu University School of Veterinary Medicine"},{"subitem_text_value":"Molecular Biology, Azabu University School of Veterinary Medicine"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"舟場, 正幸"},{"creatorName":"フナバ, マサユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"池田, 輝雄"},{"creatorName":"イケダ, テルオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村上, 賢"},{"creatorName":"アベ, マタノブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Funaba, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ikeda, Teruo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Abe, Matanobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2005-01-01"}],"displaytype":"detail","filename":"bull_jau_vol11-12-106.pdf","filesize":[{"value":"371.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol11-12-106.pdf","url":"https://az.repo.nii.ac.jp/record/4839/files/bull_jau_vol11-12-106.pdf"},"version_id":"8002059b-c185-4d15-8f06-5a30ec980e64"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アクチビンの情報伝達に関する分子機構 : 普遍的伝達と組織特異的伝達","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アクチビンの情報伝達に関する分子機構 : 普遍的伝達と組織特異的伝達"},{"subitem_title":"Molecular mechanism of activin signaling : common pathway and tissue-specific regulation","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["508"],"pubdate":{"attribute_name":"公開日","attribute_value":"2005-01-01"},"publish_date":"2005-01-01","publish_status":"0","recid":"4839","relation_version_is_last":true,"title":["アクチビンの情報伝達に関する分子機構 : 普遍的伝達と組織特異的伝達"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-06-19T08:00:54.190635+00:00"}