{"created":"2023-06-19T07:19:01.257024+00:00","id":4689,"links":{},"metadata":{"_buckets":{"deposit":"141784d5-2717-4437-9fb8-503e497ca32e"},"_deposit":{"created_by":4,"id":"4689","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4689"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004689","sets":["17:251:307","17:251:309","31:181:490"]},"author_link":["20452","20459","20454","20451","20461","20456","20460","20458","20462","20453","20455","20457"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"238","bibliographicPageStart":"237","bibliographicVolumeNumber":"9/10","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"イヌ扁平上皮がん由来細胞およびイヌ線維肉腫由来細胞からcolchicine耐性株として選択した細胞へのPgp発現をwestern blotおよび定量的PCRで確認した。各薬剤耐性株へのPgp発現が,タンパク質およびmRNAレベルで確認され,Co-PCBsのPgp影響モデルの1つが確立された。Pgp発現細胞の膜標品をN_2ガス破砕法で調製し,そのATPase活性をATP regenerating系で測定した。各阻害剤および活性化剤を用いPgp-ATPaseほかNa,K-ATPaseおよびCa-ATPase活性測定が可能であった。しかし,Pgp-ATPase活性は極めて低く,本測定法ではCo-PCBs阻害の速度論的解析は困難であると判断した。したがって,次年度はPgpを精製し,そのPgp-ATPase活性を測定することを試みることにした。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Expression of Pgp was confirmed by western blot and quantitative PCR in canine squamous cell carcinoma and canine fibrosarcoma which were selected as colchicine tolerance cell line. The protein and mRNA of Pgp were found in the cells, thus one of the examination models for the effect of Co-PCBs on Pgp was established. The membrane was prepared by N_2 disruption from Pgp expressed cells, and the ATPase was measured by ATP regeneration system. The activities of Pgp-ATPase, Na,K-ATPase and Ca-ATPase were measured by adding with the inhibitor and/or the activator. However, Pgp-ATPase activity was too small to analyze kinetics of the inhibition with Co-PCBs. Therefore, we are going to purify Pgp and measure its ATPase activity in the next year.","subitem_description_type":"Other"}]},"item_12_description_18":{"attribute_name":"レポート・演題番号","attribute_value_mlt":[{"subitem_description":"麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ2","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author 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Researcher"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"000127"},{"subitem_text_value":"000242"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"-"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤瀬, 浩"},{"creatorName":"フジセ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"荻原, 喜久美"},{"creatorName":"オギワラ, キクミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"落合, 秀治"},{"creatorName":"オチアイ, ヒデハル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"片倉, 賢"},{"creatorName":"カタクラ, ケン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"植田, 和光"},{"creatorName":"ウエダ, カズミツ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岡本, 芳春"},{"creatorName":"オカモト, ヨシハル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujise, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogiwara, Kikumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ochiai, Hideharu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katakura, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueda, Kazumitsu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Yoshiharu","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2004-01-01"}],"displaytype":"detail","filename":"bull_jau_vol9-10-237.pdf","filesize":[{"value":"156.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol9-10-237.pdf","url":"https://az.repo.nii.ac.jp/record/4689/files/bull_jau_vol9-10-237.pdf"},"version_id":"8b4d52a2-a6cc-4ac5-8345-b54396d96ded"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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