@article{oai:az.repo.nii.ac.jp:00004681,
 author = {根尾, 櫻子 and 神作, 宣男 and 古市, 満 and 渡辺, 征 and 久松, 伸 and 辻本, 元 and 久末, 正晴 and 土屋, 亮 and 山田, 隆紹 and NEO, Sakurako and KANSAKU, Norio and FURUICH, Mitsuru and WATANABE, Masashi and HISAMATSU, Sin and TSUJIMOTO, Hajime and HISASUE, Masaharu and TSUCHIYA, Ryo and YAMADA, Takatsugu},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {c-Met proto oncogeneは肝細胞増殖因子(Hepatocyte growth factor: HGF)のレセプターであり,ヒト,マウスではあらゆる上皮,内皮系細胞での発現が認められている。HGF/c-Metシグナル伝達は,細胞の増殖,運動および形態形成などを誘導し,その機能的重要性が示唆されるが,本研究ではイヌのc-Met完全長cDNAのクローニングおよび組織分布の検討を行った。イヌc-Met cDNAのOpen reading frameは4419塩基,1383個のアミノ酸で構成され,さらにmRNAは,末梢血単核球,骨髄,肝臓,腎臓,肺,胃,子宮,精巣,胸腺,リンパ節,小腸,大腸,副腎,甲状腺心臓,筋肉,皮膚,膵臓,卵巣,前立腺,脾臓,脂肪,大脳,小脳組織での発現が確認された。一方,急性骨髄性白血病(1/1),慢性骨髄性白血病(2/2),骨髄異形生症候群(1/3)の犬の骨髄でC-Met mRNAの過剰な発現が認められたのに対しリンパ球性白血病および形質細胞腫の犬の骨髄での発現は正常組織と同程度であった。, The c-Met proto-oncogene is the receptor for hepatocyte growth factor (HGF), which is a member of the tyrosine kinase family. Activation of the HGF/c-Met signal pathway leads to cell proliferation, motility, and morphogenesis. In human and mouse, c-Met is detected in various epithelial and endothelial cells and numerous studies have indicated it to be an important component of cellular function. In this study, the complete sequence of complementary DNA (cDNA) of canine c-Met was cloned, and its distribution was determined in tissues. The canine c-Met cDNA clone had an open reading frame of 4419 bp which encoded a putative polypeptide of 1383 amino acids. Furthermore, it was indicated that c-Met was expressed in a variety of canine tissues including peripheral blood mononuclear cells (PBMC), bone marrow, liver, kidney, lung, stomach, uterus, testis, thymus, lymph node, small intestine, colon, adrenal grand, thyroid gland, heart, muscle, skin, pancreas, ovary, prostate, spleen, fat, cerebrum and cerebellum. The c-Met m-RNA was overexpressed in the bone marrow cells from acute myelocytic leukemia (1/1), chronic myelocytic leukemia (2/2) and myelodysplastic syndrome (1/3), however, the expression of m-RNA in the bone marrow of chronic lymphocytic leukemia and plasmacytoma was normal bone marrow level., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {198--203},
 title = {犬の肝幹細胞に関する研究「犬c-Met/HGFレセプターcDNAのクローニングとその組織分布」},
 volume = {9/10},
 year = {2005},
 yomi = {ネオ, サクラコ and カンサク, ノリオ and フルイチ, ミツル and ワタナベ, マサシ and ヒサマツ, シン and ツジモト, ハジメ and ヒサスエ, マサハル and ツチヤ, リョウ and ヤマダ, タカツグ}
}