@article{oai:az.repo.nii.ac.jp:00004675,
 author = {滝沢, 達也 and 神作, 宜男 and 田中, 和明 and TAKIZAWA, Tatsuya and KANSAKU, Norio and TANAKA, Kazuaki},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {妊娠の維持と調節機構にNOが重要な役割を有していることが示唆されているが,NOは不安定なフリーラジカルであるため,解析が困難であった。近年,ジチオカルバメート鉄錯体であるFe-DTCS(Fe-N-(dithiocarboxy)-sarcosine)を用いて,不安定なNOを安定なNO-Fe-DTCS錯体にトラップした後,電子常磁性共鳴吸収(electron paramagnetic resonance:EPR)装置により解析することにより,NO産生を検出し,定量化できることが報告されている。本研究では,このスピントラップ-EPR法により胎盤と子宮におけるNO産生を解析し,定量的RT-PCR法によりNO産生に寄与するNOSアイソフォームを明らかにすることにより,子宮と胎盤におけるNO産生の意義とその調節機構を検討した。, Nitric oxide (NO) production in the rat uterus was quantified by electron paramagnetic resonance (EPR) spectroscopy using an iron complex with N-(dithiocarboxy) sarcosine (DTCS) as NO trapping reagents. The expression of NOS isoforms was also examined by quantitative RT-PCR analysis. The EPR spectrum of the uterus with Fe-DTCS trapping showed a triplet signal (g=2.038) derived from an NO-Fe-DTCS complex. The EPR signal was diminished in the uterus after NOS inhibitor L-NAME was administered to pregnant rats. The height of the triplet signal obtained from preterm (day 17 of gestation) uterus was significantly higher than that of term uterus (day 21). The level of iNOS mRNA expression in preterm uterus was significantly increased compared to that in term uterus, and significantly higher than that of eNOS mRNA expression in the same uterus. These results suggest that iNOS is the predominant producer of NO in preterm (day 17) uterus and that iNOS-generated NO plays significant roles in the maintenance of pregnancy., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {176--178},
 title = {妊娠動物の胎盤と子宮におけるNO産生の意義とNOS遺伝子発現調節機構},
 volume = {9/10},
 year = {2005},
 yomi = {タキザワ, タツヤ and カンサク, ノリオ and タナカ, カズアキ}
}