@article{oai:az.repo.nii.ac.jp:00004670,
 author = {和田, 恭則 and 印牧, 信行 and 恩田, 賢 and Wada, Yasunori and Kanemaki, Nobuyuki and Onda, Ken},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {周産期のウシ乳腺組織に対する1α,25(OH)_2D_3とその受容体(VDR)の関与について検討した。初産のジャージー種乳牛から分娩前後,定期的に腹大動脈と乳静脈より採血し,血清カルシウム濃度と血漿1α,25(OH)_2D_3濃度をそれぞれ測定したところ,乳腺組織前後の動静脈間でそれぞれの濃度に,大きな差を認めなかった。VDRのcDNA部分クローニングから推測されるホルスタイン種のアミノ酸配列は,ホルモン結合部位と考えられる部位でジャージー種と違いが大きかった。また,妊娠および泌乳期でも乳腺組織でのVDR遺伝子の発現は低く,著しい増加は観察できなかった。さらに,ウシ腎臓由来の株化細胞である,MDBKとCKT-1では,PMAの刺激でVDR遺伝子の発現が増加することが分かった。, The purpose of this study was to identify the importance of 1α, 25(OH)_2D_3 and its receptor (VDR) in periparturient mammary gland of cattle. Blood samples were collected from a periparturient Jersey cattle regularly. Concentrations of serum calcium and plasma 1α, 25(OH)_2D_3 in abdominal aorta and suscutaneous veins of abdomen were not obvious differences from 25 days before to 115 days after parturition. Partial cDNA cloning of Holstein VDR defined that deduced amino acid sequences in Holstein cattle have several differences that of in Jersey cattle at estimated hormone binding site in humans. VDR gene expressions in the mammary gland of a periparturient cattle were very low and the rapid increase of VDR gene expression which related to pregnant and lactation could not observe in this study. Furthermore, PMA stimulation increased VDR gene expressions in MDBK and CKT-1, both cell lines were derived from the bovine kidney., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {158--160},
 title = {ウシ乳熱に対する1α,25(OH)_2D_3受容体の関与},
 volume = {9/10},
 year = {2005},
 yomi = {ワダ, ヤスノリ and カネマキ, ノブユキ and オンダ, ケン}
}