| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2004-01-01 |
| タイトル |
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タイトル |
微小管結合タンパク質タウの神経細胞死における役割に関する研究 |
| タイトル |
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タイトル |
Role of microtubule associated protein tau in neuronal cell death |
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言語 |
en |
| 言語 |
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言語 |
jpn |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
| 記事種別 |
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内容記述タイプ |
Other |
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内容記述 |
特集 |
| Type |
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内容記述タイプ |
Other |
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内容記述 |
FEATURE ARTICLES |
| 著者 |
村山, 洋
岩橋, 和彦
秋山, 孝洋
松田, 基夫
Murayama, Ohoshi
Iwahashi, Kazuhiko
Takahiro, Akiyama
Matsuda, Motoo
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| 著者ID |
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000298 |
| 著者ID |
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000278 |
| 著者ID |
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000235 |
| 著者ID |
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000135 |
| Author ID |
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000298 |
| Author ID |
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000278 |
| Author ID |
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000235 |
| Author ID |
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000135 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| 所属機関 |
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麻布大学大学院環境保健学研究科 |
| Institution or Company |
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The Graduate School of Environmental Health Sciences, Azabu University |
| Institution or Company |
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The Graduate School of Environmental Health Sciences, Azabu University |
| Institution or Company |
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The Graduate School of Environmental Health Sciences, Azabu University |
| Institution or Company |
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The Graduate School of Environmental Health Sciences, Azabu University |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
神経原線維変化の形成に重要な関わりを持つタウタンパク質の2つの型のアイソフォーム(3リピートタウと4リピートタウ)のmRNAレベルを識別定量する条件を検討した。本法は,神経細胞変性過程におけるタウ遺伝子発現およびアイソフォーム発現パターンの動態を追跡する上で有効な手段であると考えられる。 |
| Abstract |
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内容記述タイプ |
Other |
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内容記述 |
Neurofibrillary tangle composed of hyperphosphorylated tau aggregation in neuron is one of major pathological hallmarks of Alzheimer's disease or other neurodegenerative disease. Recently, familial mutations in tau gene were identified by reverse genetic analysis of FTDP-17 families. Some of these mutation lead amino acid substitution and the other affect alternative splicing of the exon10. This suggests that tau is possibly involved in developing "tauopathy". Although tau is known as an axonal microtubule associated protein that stabilized the structure solubility of microtubules and promotes polymerization of tubulines into the microtubules, role(s) of tau in pathological events remains unclear. To elucidate how tau protein and its isoforms are involved in the neurodegeneration, we developed quantitative method for tau isoforms, three and four repeat tau, respectively. Real Time PCR using Taq Man probe (ABI) three and four repeat tau mRNA could be quantified with high sensitivity (> 10pg of total RNA). In addition, a little cross reactivity between three and four repeat tau mRNA was observed. These results suggested that tau mRNA in small number of neuronal cells (～10 cells) of mouse or human brain could be quantified by our method. Combining with isoform specific antibodies T3R and T4R previously generated, the measurement may be useful for studying the molecular mechanism in developing dementia. |
| 書誌情報 |
麻布大学雑誌
en : Journal of Azabu University
巻 9/10,
p. 146-147,
発行日 2005-03-31
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| 出版者 |
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出版者 |
麻布大学 |
| Publisher |
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出版者 |
Azabu University |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346-5880 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11561468 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| text version |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| format |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
bull_jau_vol9-10-146.pdf (183.9 kB)
