{"created":"2023-06-19T07:19:00.220421+00:00","id":4665,"links":{},"metadata":{"_buckets":{"deposit":"ab462728-3555-44c4-a44f-60f03e001bd7"},"_deposit":{"created_by":4,"id":"4665","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4665"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004665","sets":["17:253:311","17:254:339","17:38:264","17:40:281","31:181:490"]},"author_link":["20265","20260","20258","20266","20259","20257","20264","20262","20261","20263"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"140","bibliographicPageStart":"136","bibliographicVolumeNumber":"9/10","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"乳酸菌のプロバイオティクス性を示す指標の一つとして腸管上皮細胞への付着性があげられる。乳酸菌に蛍光タンパク質遺伝子を発現させ,産生されたタンパク質の蛍光をマーカーとすることを試みた。まずE. coliのプロモーターに連結された蛍光タンパク質遺伝子(pECFP, pEYFP)を乳酸菌で発現させるために,pIL253をベースに乳酸菌用プロモーター領域およびE. coli用oriを組み込んだシャトルベクターpKTを作成した。次に蛍光タンパク質遺伝子ECFPとEYFPをそれぞれpKTに組み込んだpKTCFPおよびpKTYFPを作成し,Lactobacillus reuteriを形質転換した。その結果,pKTCFPによる形質転換体で蛍光タンパク質が産生され,蓄積された蛍光タンパク質により発光するL. reuteriの形質転換体を得ることができた。黄色の蛍光タンパク質に関しては,pKTYFPよりpH抵抗性の高いpKTEYFP-L68V/Q69Kを構築し,一般の培養でも黄色の蛍光色を発するL. reuteriの形質転換体の獲得に成功した。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Adherence of lactic acid bacteria (LAB) to intestinal epithelial cells is one of the important characteristics as probiotics. We tried to express the fluorescence protein genes ECFP (cyan fluorescence) or EYFP (yellow fluorescence) in LAB. The fluorescence was used as a marker of LAB adherence to intestinal epithelial cells. A shuttle vector pKT between E. coli and LAB was constructed the origin of DNA replication from pMB1 for E. coli and pIL253, which is a cloning vector for LAB. pKTCFP and pKTYFP were introduced fluorescence protein genes ECFP or EYFP from commercial pECFP or pEYFP, respectively. L. reuteri was transformed by pKTCFP produced cyan fluorescence protein and fluorescence was detected from this bacterium. Furthermore, pH resistant yellow fluorescence protein gene, pKTEYFP-L68V/Q69K, was constructed, and yellow fluorescence was detected from the transformant of L. reuteri with this plasmid.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author 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University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000247"},{"subitem_text_value":"000395"},{"subitem_text_value":"-"},{"subitem_text_value":"000075"},{"subitem_text_value":"-"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 英利"},{"creatorName":"モリタ, ヒデトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"20257","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木, 武人"},{"creatorName":"スズキ, タケヒト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"20258","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平戸, 久留実"},{"creatorName":"ヒラト, クルミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"20259","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤, 行男"},{"creatorName":"カトウ, ユキオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"20260","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"滝沢, 達也"},{"creatorName":"タキザワ, タツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"20261","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Morita, Hidetoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20262","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Takehito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20263","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirato, Kurumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20264","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Yukio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20265","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takizawa, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20266","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2004-01-01"}],"displaytype":"detail","filename":"bull_jau_vol9-10-136.pdf","filesize":[{"value":"721.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol9-10-136.pdf","url":"https://az.repo.nii.ac.jp/record/4665/files/bull_jau_vol9-10-136.pdf"},"version_id":"f857b297-7f01-4d5e-97f5-3f9616e34ae2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Lactobacillus属乳酸菌での蛍光タンパク発現ベクター系の構築とその応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Lactobacillus属乳酸菌での蛍光タンパク発現ベクター系の構築とその応用"},{"subitem_title":"Construction of expression vector system and application of the fluorescence protein genes in lactic acid bacteria (lactobacilli)","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["264","281","311","339","490"],"pubdate":{"attribute_name":"公開日","attribute_value":"2004-01-01"},"publish_date":"2004-01-01","publish_status":"0","recid":"4665","relation_version_is_last":true,"title":["Lactobacillus属乳酸菌での蛍光タンパク発現ベクター系の構築とその応用"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:48:27.030373+00:00"}