@article{oai:az.repo.nii.ac.jp:00004456,
 author = {猪股, 智夫 and Inomata, Tomoo},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {妊娠10日目のMuta mouse(大腸菌のLacZ遺伝子をトランスジェニックしたマウス)にPCB126を500μg/kg,対照群にはコーンオイルを単回経口投与し,妊娠18日目に胎子を取り出し,催奇形性の有無および胎子DNA(LacZ遺伝子)の遺伝子突然変異頻度を調べた。また,自然分娩させたものにつては,膣開口日を観察し,性成熟した時点で,自発運動量センサーを用いて夜間の行動量を測定した。その結果,PCB126は,マウス胎子に口蓋裂を誘発したが,これに伴う遺伝子突然変異誘発性は殆ど認められなかった。しかし,PCB126は次世代マウスの雌における性成熟を早めるとともに,夜間の行動量を増加させた。, PCBs are persistent environmental agents that show endocrinological and immunological impairments and tumorgenic and teratogenic effects. In this study we used the transgenic mouse model (Muta Mouse), carrying bacterial lacZ genes, to assess the effects of 3,3',4,4',5- pentachlorobiphenl (PCB126) on the prenatal and postnatal offspring. Mothers on the 10th day of pregnancy were given a single injection of PCB126 (500 micrograms/kg) orally. Mothers on the 18th day of pregnancy were sacrificed by decapitation to obtain their fetuses. The fetuses were subjected to observe their external malformations and gene mutations. The other mothers delivered and their offspring were subjected to observe the period of vaginal opening and to measure their motor activity. Fetuses of experimental group showed low incidental rates of the external malformation, cleft palate (5%). DNA (LacZ gene) extracted from these fetuses especially in malformed fetuses showed low incidents of LacZ mutation, 4.11×10-<-5> which was mostly the same as controls, 2.79×10^<-5>. The periods of vaginal opening in the pups of experimental group were little earlier than that observed in the controls. The amounts of motor activity in the pups of experimental group were higher than that observed in the controls. The results indicate that PCB126 induced the malformation and gene mutations in the fetuses but it's incidental rates were low levels, and that PCB126 induces to accelerate the sexual maturation of the female pups and to increase the amounts of the motor activity., 麻布大学ハイテク・リサーチ・センター研究プロジェクト 研究サブ・グループ4, P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {211--212},
 title = {トランスジェニックマウスを用いたコプラナーPCSsの毒性評価},
 volume = {5/6},
 year = {2003}
}