{"created":"2023-06-19T07:18:50.344101+00:00","id":4447,"links":{},"metadata":{"_buckets":{"deposit":"34682e47-6d87-4a26-abb5-56d803113713"},"_deposit":{"created_by":4,"id":"4447","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4447"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004447","sets":["17:236:244","17:254:334","17:42:292","31:181:487"]},"author_link":["19440","19447","19448","19444","19439","19441","19443","19446","19442","19445"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"186","bibliographicPageStart":"182","bibliographicVolumeNumber":"5/6","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"近年,種々の組織において,多能性幹細胞の存在が明らかにされている。例えば,骨髄細胞は骨髄細胞のみならず骨格筋,血管内皮細胞,神経細胞,肝細胞への分化が可能である。そして,これらの細胞が他の組織細胞に分化するためには,様々なサイトカインが必要である。ラットの骨髄細胞はHGF存在下で肝臓の細胞に分化することが報告され,このHGFは,あらゆる組織の細胞表面のレセプターであるc-Metチロシンキナーゼを介してあらゆる組織において組織再構築の為の生物活性を誘導するサイトカインの一つである。HGFの機能を検討する為に,我々はイヌc-Met遺伝子のクローニングを行い,また各組織での発現をRT-PCRにより検討した。現在までに80%のシークエンス解析が終了しており,すでに部分配列である724bPをGenbankに登録した。\nさらに,骨髄細胞が肝細胞に分化する可能性を検討するために,骨髄細胞をHGF(30ng/ml)存在下で培養した。その後,培養骨髄細胞はRT-PCRによって肝細胞のマーカーであるAFP, Albuminの発現について検討した。しかし,これらの遺伝子の発現は確認できず,今回の研究で用いたHGFの量は,骨髄細胞が肝細胞に分化するのに十分量ではなかったと考えられた。次回の研究では高濃度HGF下でイヌ骨髄細胞を培養し肝細胞への分化を証明したいと考えている。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Recently, pluripotential stem cells are observed in various tissues. For example, bone marrow cells can differentiate into not only hematopoietic stem cell but also to other tissues, like as cardiac muscles, vascular endothelial cells, neurocytes and hepatocytes. For these cells' differentiation, a number of cytokines are needed. Rat bone marrow cells can differentiate into hepatocytes by culture with hepatocyte growth factor (HGF) containing medium. HGF is the one of these cytokines which induces the biological activation in every tissues to reconstruct the tissue through c-Met tyrosine, which is cell surface receptor for HGF and mediate the biological activity of the HGF. To investigate HGF function, we cloned complementary DNA for canine c-Met and analyzed its expression in some tissues by reverse transcriptase PCR (RT-PCR) amplification. Eighty % of the whole sequence have been sequenced and we subscribed the partial code (724bp) to GenBank. \nFurthermore, to determine the probability that bone marrow cells differentiate to hepatocytes, we cultured fresh canine bone marrow cells with HGF (30ng/ml). Then the cultured cells were examined by RT-PCR the expression of mRNA of albumin and AFP as the marker of hepatocyte. Canine bone marrow cells did not express the hepatocyte marker under culture condition with 30 ng/ml HGF containing medium. This study indicates that the concentration of HGF was not enough for bone marrow cells to defferentiate into hepatocytes. Consequently, we intend to culture bone marrow cells with medium containing high concentration of HGF at next experiments.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"000396"},{"subitem_text_value":"000317"},{"subitem_text_value":"000071"},{"subitem_text_value":"-"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院獣医学研究科"},{"subitem_text_value":"麻布大学大学院獣医学研究科"},{"subitem_text_value":"麻布大学獣医学部"},{"subitem_text_value":"麻布大学大学院獣医学研究科"},{"subitem_text_value":"東京大学大学院農学生命科学研究科"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Veterinary Science, Azabu University"},{"subitem_text_value":"Graduate School of Veterinary Science, Azabu University"},{"subitem_text_value":"School of Veterinary Medicine, Azabu University"},{"subitem_text_value":"Graduate School of Veterinary Science, Azabu University"},{"subitem_text_value":"Graduate School of Agricultural and Life Sciences. The University of Tokyo"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"-"},{"subitem_text_value":"000396"},{"subitem_text_value":"000317"},{"subitem_text_value":"000071"},{"subitem_text_value":"-"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山田, 隆紹"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"根尾, 櫻子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"久末, 正晴"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"土屋, 亮"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"辻本, 元"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Takatsugu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Neo, Sakurako","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hisasue, Masaharu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuchiya, Ryo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsujimoto, Hajime","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2002-01-01"}],"displaytype":"detail","filename":"bull_jau_vol5-6-182.pdf","filesize":[{"value":"467.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol5-6-182.pdf","url":"https://az.repo.nii.ac.jp/record/4447/files/bull_jau_vol5-6-182.pdf"},"version_id":"07335e0b-2cff-4b4b-9eae-90bcd756f241"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"犬肝幹細胞に関する研究 : 犬c-Met遺伝子のクローニング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"犬肝幹細胞に関する研究 : 犬c-Met遺伝子のクローニング"},{"subitem_title":"Studies on canine hepatic stem cells : Cloning of canine c-Met gene","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["244","292","334","487"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-01-01"},"publish_date":"2002-01-01","publish_status":"0","recid":"4447","relation_version_is_last":true,"title":["犬肝幹細胞に関する研究 : 犬c-Met遺伝子のクローニング"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:48:11.087093+00:00"}