{"created":"2023-06-19T07:18:50.253763+00:00","id":4445,"links":{},"metadata":{"_buckets":{"deposit":"26029abb-9726-46d1-81d6-121e06a4f476"},"_deposit":{"created_by":4,"id":"4445","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4445"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004445","sets":["17:40:278","17:40:287","31:181:487"]},"author_link":["19425","19428","19424","19429","19430","19427","19423","19426"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"175","bibliographicPageStart":"173","bibliographicVolumeNumber":"5/6","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究においてデザインされた,recA遺伝子のほぼ全長に渡る領域を増幅するためのPCRプライマーを用いて得られたPCR産物の配列情報は,UPTC及び他の高温性カンピロバクターのDNAレベルでの分子識別に大変有用であることが強く示唆された。更に,本研究の主目的であるUPTCのゲノムDNA上の多遺伝子配列情報に基づく分子識別にrecA遺伝子を対象とすることは極めて有用であることが初めて明らかとなった。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"In the present study, for the first time, nucleotide sequencing after TA cloning of almost the full-length (about 1,050 bp in length) of the recA gene of two isolates of UPTC, NCTC12894 and CF89-12 and a strain of C. lari JCM2530T amplified using a degenerate primer pair was carried out. Nucleotide sequence comparison analysis of the possible open reading frame (ORF) demonstrated that the two isolates of UPTC showed about 94% sequence homology of the recA gene to each other and that they also showed 90-92% and about 84% homology of the recA gene to C. lari and C. jejuni, respectively. The two isolates of UPTC showed 50-60% homology of the recA gene to the other family Enterobacteriaceae. \nIn conclusion, this study presents novel sequence data on recA for C. lari and UPTC, which may aid in the phylogenetic positioning of the UPTC group within the genus Campylobacter and may aid in the discriminating of isolates of the UPTC group.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000135"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000298"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院環境保健学研究科"},{"subitem_text_value":"順天堂大学大学院医学研究科"},{"subitem_text_value":"麻布大学大学院環境保健学研究科"},{"subitem_text_value":"麻布大学大学院環境保健学研究科"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Environmental Health Sciences, Azabu University"},{"subitem_text_value":"Graduate School of Medicine, Juntendo University"},{"subitem_text_value":"Graduate School of Environmental Health Sciences, Azabu University"},{"subitem_text_value":"Graduate School of Environmental Health Sciences, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000135"},{"subitem_text_value":"-"},{"subitem_text_value":"-"},{"subitem_text_value":"000298"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松田, 基夫"}],"nameIdentifiers":[{"nameIdentifier":"19423","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高宮, 信三郎"}],"nameIdentifiers":[{"nameIdentifier":"19424","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"三田, 明弘"}],"nameIdentifiers":[{"nameIdentifier":"19425","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村山, 洋"}],"nameIdentifiers":[{"nameIdentifier":"19426","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuda, Motoo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19427","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takamiya, Shinzaburo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19428","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sanda, Akihiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19429","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murayama, Ohoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"19430","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2002-01-01"}],"displaytype":"detail","filename":"bull_jau_vol5-6-173.pdf","filesize":[{"value":"255.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol5-6-173.pdf","url":"https://az.repo.nii.ac.jp/record/4445/files/bull_jau_vol5-6-173.pdf"},"version_id":"b83a160e-f81d-42f7-b773-91b67c250efb"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"新しい細菌,ウレアーゼ陽性高温性カンピロバクターのゲノムDNA上の多遺伝子配列情報に基づく分子識別 ; recA遺伝子の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新しい細菌,ウレアーゼ陽性高温性カンピロバクターのゲノムDNA上の多遺伝子配列情報に基づく分子識別 ; recA遺伝子の解析"},{"subitem_title":"Molecular discrimination of newly identified eubacterium, urease-positive thermophilic campylobacter (UPTC) based on the sequence information of multiple genes on the genomic DNA; analysis of recA gene","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["278","287","487"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-01-01"},"publish_date":"2002-01-01","publish_status":"0","recid":"4445","relation_version_is_last":true,"title":["新しい細菌,ウレアーゼ陽性高温性カンピロバクターのゲノムDNA上の多遺伝子配列情報に基づく分子識別 ; recA遺伝子の解析"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:49:59.306985+00:00"}