@article{oai:az.repo.nii.ac.jp:00004438,
 author = {藤谷, 英男 and 藤瀬, 浩 and 若尾, 義人 and Fujitani, Hideo and Fujise, Hiroshi and Wakao, Yoshito},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {1.16S rDNA(一部)の塩基配列を解析することにより,チーターに感染するH属菌が少なくとも4種類あることがわかり,PCR-RFLP解析により,それらを3つのグループに分けることが可能であった。ブタでは少なくとも3種類のH属菌の感染が確認でき,胃内での存在部位により菌種が異なっていることがわかった。2.PCRは糞便サンプルからのCPVの検出および型判別に有効であることを確認した。まず,CPV-2型かCPV-2bまたは-2bかを検出し,後者だった場合は,さらにCPV-2bの判別をすることが可能だった。4.DNA鑑定により,本個体は通常の雌と同様にXX型染色体を示すが,雄特有のSRY遺伝子ももっている(おそらく減数分裂時の組換えによる)ことから半陰陽になったと考えられた。, Four independent studies were carried out. They were: 1. Species identification by genome analyses of the genus Helicobacter that infected the cheetah, the swine and the monkey; 
2. Usefulness of PCR-detection of viral genes in fecal samples from canine parvovirus infected dogs;
3. Sexing of birds by DNA analyses; and 4. DNA-sexing of a case of hermaphroditism found in a miniature dachshund. Results from each study will be described separately. 
1. Helicobacter Nucleotide sequence homology was examined for PCR-amplified segment of 16S rDNA to differentiate the Helicobacter species. The cheetah samples were classified into four species, including the closely related Flexispira rappini. On the basis of restriction site distribution, these were more conveniently grouped into three RFLP criteria, one consisting of the two Helicobacter species. Three Helicobacter species were identified in swine specimens, one at the fundus or pylorus, and two at the cardia. Although the three species from simian samples were highly homologous to the human bacteria, they were considered to be unique to monkeys after phylogenetic analysis. 
2. Canine parvoviras (CPV) Fecal samples proved to be convenient and satisfactoty materials for differential PCR detection of CPV. With the first step primers CPV was divided into CPV-2 and a combination of its two subtypes. The second set of primers further divided the latter into CPV-2a and CPV-2b, thus clearly identifying the three known antigenic types of CPV by using dog feces. 
3. Avian sexing The avian sex chromosomes (W and Z) carries chromobox helicase-DNA binding (CHD) protein genes with its intron differing in length by sex. Taking advantage of the differences of this and the nucleotide sequence of sex chromosome-linked EE 0.6 region, the two PCR methods successfully identified snowy owl's sex (using blood sample), and the CHD-PCR, chicken's (using feather). 4. Sexing of a hermaphroditic dog The highly accurate PCR/RFLP method we previously reported was applied to sexing of a case of hermaphroditic miniature dachshund that possessed a mixed external and internal genitalia. DNA from its nail showed that this dog had XX chromosome set and (essentially Y-specific) SRY sequence. It was concluded that this originally female dog had somehow acquired SRY segment, presumably during meiosis in one of its parents., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {150--153},
 title = {DNA解析による動物の感染症の確定診断と鳥類の性別},
 volume = {5/6},
 year = {2003}
}