{"created":"2023-06-19T07:18:49.815959+00:00","id":4435,"links":{},"metadata":{"_buckets":{"deposit":"39e2ba56-535c-44ef-aa01-6ce81b54e893"},"_deposit":{"created_by":4,"id":"4435","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"4435"},"status":"published"},"_oai":{"id":"oai:az.repo.nii.ac.jp:00004435","sets":["17:254:339","17:257:356","31:181:487"]},"author_link":["19367","19368","19365","19366"],"item_12_biblio_info_16":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"138","bibliographicPageStart":"134","bibliographicVolumeNumber":"5/6","bibliographic_titles":[{"bibliographic_title":"麻布大学雑誌"},{"bibliographic_title":"Journal of Azabu University","bibliographic_titleLang":"en"}]}]},"item_12_description_13":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"妊娠の維持とその調節機構にNOが重要な役割を有していることが示唆されているが,NOは不安定なフリーラジカルであるため,解析が困難であった。近年,ジチオカルバメート鉄錯体であるFe-DTCS(Fe-N-(dithiocarboxy) sarcosine)を用いて,不安定なNOを安定なNO-Fe-DTCS錯体にトラップした後,電子常磁性共鳴吸収(electron paramaenetic resonance:EPR)装置により解析することにより,NO産生を検出し,定量化できることが報告されている。本研究では,このスピントラップ-EPR法により胎盤と子宮におけるNO産生を解析し,定量的RT-PCR法によりNO産生に寄与するNOSアイソフォームを明らかにすることにより,妊娠後期の胎盤と子宮におけるNO産生の意義とその調節機構を検討した。\n妊娠19.5日から妊娠21.8日の胎盤と子宮におけるNO産生に変動は認められなかった。定置的RT-PCR法により,妊娠21.5日の胎盤においてはiNOSmRNAの発現が妊娠19.5日と比べて有意に増加していたが,eNOSmRNAの発現に妊娠時期による変動は認められなかったことから,妊娠後期の胎盤におけるNO産生には,eNOSも関与しているものの,iNOSの寄与が大きいものと考えられた。以上の結果から,妊娠末期の胎盤においては,血小板凝集抑制や血流量維持などに関与しているNO産生を分娩直前まで維持するために,iNOS遺伝子の発現が転写レベルで調節されているものと考えられた。","subitem_description_type":"Abstract"}]},"item_12_description_14":{"attribute_name":"Abstract","attribute_value_mlt":[{"subitem_description":"Nitric oxide (NO) production in the rat placenta and uterus was monitored and quantified by electron paramagnetic resonance (EPR) spectroscopy using an iron complex with N-(dithiocarboxy) sarcosine (Fe-DTCS) as NO trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative reverse transcriptase-mediated polymerase chain reaction (RT-PCR) analysis. The EPR spectrum of the placenta with Fe-DTCS trapping showed a triplet signal (g=2.038) derived from an NO-Fe-DTCS complex. The heights of NO-Fe-DTCS (a) and MnO (b) signal were simultaneously measured to calculate the ratio (a/b) of these signal heights as used for the quantification of the NO production level. The ratio of signal heights of NO-Fe-DTCS and MnO did not vary significantly with gestational stage in the placenta and uterus during the last few days of gestation. At the gestational stages examined, the level of NOS II (iNOS) mRNA expression was significantly higher than of NOS III (eNOS) mRNA expression at a given stage in the placenta. NOS II expression in term (day 21.5) placenta was significantly increased compared to that in preterm (day 19.5) placenta (p<0.01, n=4 or 5). These results suggest that NOS II is the predominant producer of NO in the placenta and that NOS II-generated NO plays significant roles in the maintenance of placental functions immediately before birth.","subitem_description_type":"Other"}]},"item_12_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_12_description_3":{"attribute_name":"記事種別","attribute_value_mlt":[{"subitem_description":"特集","subitem_description_type":"Other"}]},"item_12_description_34":{"attribute_name":"format","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_12_description_4":{"attribute_name":"Type","attribute_value_mlt":[{"subitem_description":"FEATURE ARTICLES","subitem_description_type":"Other"}]},"item_12_publisher_19":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"麻布大学"}]},"item_12_publisher_20":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"Azabu University"}]},"item_12_source_id_21":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-5880","subitem_source_identifier_type":"ISSN"}]},"item_12_source_id_23":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11561468","subitem_source_identifier_type":"NCID"}]},"item_12_text_10":{"attribute_name":"Author ID","attribute_value_mlt":[{"subitem_text_value":"000244"},{"subitem_text_value":"000059"}]},"item_12_text_11":{"attribute_name":"所属機関","attribute_value_mlt":[{"subitem_text_value":"麻布大学大学院獣医学研究科"},{"subitem_text_value":"麻布大学大学院獣医学研究科"}]},"item_12_text_12":{"attribute_name":"Institution or Company","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Veterinary Science, Azabu University"},{"subitem_text_value":"Graduate School of Veterinary Science, Azabu University"}]},"item_12_text_9":{"attribute_name":"著者ID","attribute_value_mlt":[{"subitem_text_value":"000244"},{"subitem_text_value":"000059"}]},"item_12_version_type_29":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_12_version_type_30":{"attribute_name":"text version","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"滝沢, 達也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山本, 雅子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takizawa, Tatsuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Masako","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2002-01-01"}],"displaytype":"detail","filename":"bull_jau_vol5-6-134.pdf","filesize":[{"value":"444.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"bull_jau_vol5-6-134.pdf","url":"https://az.repo.nii.ac.jp/record/4435/files/bull_jau_vol5-6-134.pdf"},"version_id":"729a4d59-3dd2-431a-8383-075b966d0616"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"胎盤および子宮における一酸化窒素(NO)産生の意義とその調節機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"胎盤および子宮における一酸化窒素(NO)産生の意義とその調節機構"},{"subitem_title":"Expression of nitric oxide synthase isoforms and detection of nitric oxide in term rat placenta and uterus : Possible role of NO on placental functions","subitem_title_language":"en"}]},"item_type_id":"12","owner":"4","path":["339","356","487"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-01-01"},"publish_date":"2002-01-01","publish_status":"0","recid":"4435","relation_version_is_last":true,"title":["胎盤および子宮における一酸化窒素(NO)産生の意義とその調節機構"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-06-19T07:47:25.950229+00:00"}