@article{oai:az.repo.nii.ac.jp:00004433,
 author = {村山, 洋 and 岩橋, 和彦 and 松田, 基夫 and Murayama, Ohoshi and Iwahashi, Kazuhiko and Matsuda, Motoo},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {神経原線維変化の形成に重要な関わりを持つタウタンパク質の2つの型のアイソフォーム(3リピートタウと4リピートタウ)の識別を可能にする抗体T3RおよびT4Rを得た。今回作製した抗体は,他のグループが作成したものと比較して特異性が高いことに加え,タウの微小菅結合領域の高次構造の変化を検出するのに利用可能であることが明らかとなった。微小管結合領域はタウの機能に重要な部分であり,抗体T3RおよびT4Rは痴呆症発症におけるタウの関わりを詳細に解析する上で利用価値の高い抗体だと考えられる。, Neurofibrillary tangle is one of major pathological hallmarks of Alzheimer's disease. In this pathological change, abnormal intracellular aggregates composed of hyperphosphorylated tau proteins are observed. Recently, familial missense mutations in tau gene were identified by reverse genetic analysis of FTDP-17 families. This suggests that tau is possibly involved in developing "tauopathy". Although tau is known as an axonal microtubule associated protein that stabilized the structure solubility of microtubules and promotes polymerization of tubulines into the microtubules, role (s) of tau in pathological events remains unclear. 
To elucidate role (s) of tau in the neurodegeneration, we made antibodies (T3R and T4R) recognizing specifically tau isoforms, three and four repeat tau, respectively. By enzyme linked immunosolvent assay and western blot analysis, it was revealed that the affinities of these antibodies were depend on coformations of their epitopes. In addition, a C-terminal portion of tau contributed to forming tertiary structure of these epitopes. T3R and T4R might be the biochemical markers for monitoring the change in local conformation of the repeat domain. T3R and T4R generated in this study may be powerful probe for the biochemical and pathological diagnostics of dementia., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {129--131},
 title = {神経細胞変性における微小管結合蛋白質タウのリン酸化および代謝に関する研究},
 volume = {5/6},
 year = {2003}
}