@article{oai:az.repo.nii.ac.jp:00004430,
 author = {藤瀬, 浩 and 藤谷, 英男 and 内藤, 博之 and Fujise, Hiroshi and Fujitani, Hideo and Naito, Hiroyuki},
 journal = {麻布大学雑誌, Journal of Azabu University},
 month = {Mar},
 note = {guanylinおよびuroguanylinによるNaおよびCI輸送制御測定系の検討のために,赤血球および上皮細胞を用いた。イヌ,クマ,オットセイ赤血球を用いて,Na/CaおよびNa/H交換輸送測定系,ならびにその交換輸送による細胞容積収縮および膨潤調節測定系を確立した。上皮細胞によるイオン輸送測定系として,フイルター底のTranswell上に腎由来細胞であるLLC-PK1の単層細胞層を調製し,この経上皮細胞層輸送測定系を用いて頂側膜と基底膜間の水輸送を測定した。Ussing chamberに装着したマウス腸管の管腔測へのuroguanylin添加によりCIのshort-circuitcurrentが変化することを確認した。また,guanylinおよびuroguanylinのイオン輸送への役割を説明するため,研究の現状を紹介した。, To examine regulation system of Na and Cl transport by guanylin and uroguanylin, red blood cells and epithelial cells were used. In dog, bear and northern furseal red blood cells, Na transports by Na/Ca and Na/H exchangers were measured, and regulatory volume decrease and increase by these exchangers were also measured. As the ion and water transport measurement system in epithelial cells, the monolayer cells cultured with the kidney cell line, LLC-PK1, were prepared in bottom filterrd well, and transepithelial water transport was measured between apical and basal membrane. Using ussing chamber, Cl short-circuit current in mouse intestinal tract was measured, and the current was changed when uroguanylin was added at the luminal side. In explanation for the rule of guanylin and uroguanylin on these ion transports, the structure and function of these peptides were reviewed shortly., P(論文), 特集, application/pdf, FEATURE ARTICLES},
 pages = {113--118},
 title = {生体膜陽イオン輸送のグアニリン・ウログアニリン系による制御 : 中間報告 : 各種動物赤血球と上皮細胞を用いたNaあるいはCI輸送測定法の検討},
 volume = {5/6},
 year = {2003}
}